Rabbit anti-Human, Mouse POLH Polyclonal Antibody | anti-POLH antibody

POLH Polyclonal Antibody

Cat. Num. AAA8531338

• Gene Names: POLH; XPV; XP-V; RAD30; RAD30A
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: POLH; Polyclonal Antibody; POLH Polyclonal Antibody; DNA polymerase eta; EC 2.7.7.7; RAD30 homolog A; Xeroderma pigmentosum variant type protein; RAD30; RAD30A; XPV; anti-POLH antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
• Sequence Length: 414

• Applicable Applications for anti-POLH antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500-2000; ELISA: 1:5000-20000
• Preparation and Storage: Store at-20 degree C for 1 year.

NCBI and Uniprot Product Information

• NCBI GI #: 634743306
• NCBI GeneID: 5429
• NCBI Accession #: NP_001278899.1
• NCBI GenBank Nucleotide #: NM_001291970.1
• UniProt Accession #: Q9Y253; Q7L8E3; Q96BC4; Q9BX13
• Molecular Weight: 78
• NCBI Official Full Name: DNA polymerase eta isoform 3
• NCBI Official Synonym Full Names: DNA polymerase eta
• NCBI Official Symbol: POLH
• NCBI Official Synonym Symbols: XPV; XP-V; RAD30; RAD30A
• NCBI Protein Information: DNA polymerase eta
• UniProt Protein Name: DNA polymerase eta
• Protein Family: DNA polymerase
• UniProt Gene Name: POLH
• UniProt Synonym Gene Names: RAD30; RAD30A; XPV

NCBI Description

This gene encodes a member of the Y family of specialized DNA polymerases. It copies undamaged DNA with a lower fidelity than other DNA-directed polymerases. However, it accurately replicates UV-damaged DNA; when thymine dimers are present, this polymerase inserts the complementary nucleotides in the newly synthesized DNA, thereby bypassing the lesion and suppressing the mutagenic effect of UV-induced DNA damage. This polymerase is thought to be involved in hypermutation during immunoglobulin class switch recombination. Mutations in this gene result in XPV, a variant type of xeroderma pigmentosum. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2014]

Uniprot Description

DNA polymerase specifically involved in the DNA repair by translesion synthesis (TLS) (PubMed:10385124, PubMed:11743006, PubMed:24449906). Due to low processivity on both damaged and normal DNA, cooperates with the heterotetrameric (REV3L, REV7, POLD2 and POLD3) POLZ complex for complete bypass of DNA lesions. Inserts one or 2 nucleotide(s) opposite the lesion, the primer is further extended by the tetrameric POLZ complex. In the case of 1,2-intrastrand d(GpG)-cisplatin cross-link, inserts dCTP opposite the 3' guanine (PubMed:24449906). Particularly important for the repair of UV-induced pyrimidine dimers (PubMed:10385124, PubMed:11743006). Although inserts the correct base, may cause base transitions and transversions depending upon the context. May play a role in hypermutation at immunoglobulin genes (PubMed:11376341, PubMed:14734526). Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have any lyase activity, preventing the release of the 5'-deoxyribose phosphate (5'-dRP) residue. This covalent trapping of the enzyme by the 5'-dRP residue inhibits its DNA synthetic activity during base excision repair, thereby avoiding high incidence of mutagenesis (PubMed:14630940). Targets POLI to replication foci (PubMed:12606586).