RPL5 Antibody | anti-RPL5 antibody

RPL5 Antibody

Cat. Num. AAA8514442

• Gene Names: RPL5; L5; uL18; MSTP030; PPP1R135
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Immunogen affinity purified
• Synonyms: RPL5; RPL5 Antibody; 60S ribosomal protein L5; RL5; anti-RPL5 antibody

• Reactivity: Human, Mouse, Rat
• Specificity: RPL5 Antibody detects endogenous levels of total RPL5
• Purity/Purification: Immunogen affinity purified
• Form/Format: In phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 297

• Applicable Applications for anti-RPL5 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthesized peptide
• Preparation and Storage: Store at-20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from HepG2 cells and Jurkat cells, using RPL5 antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 14591909
• NCBI GeneID: 6125
• NCBI Accession #: NP_000960.2
• NCBI GenBank Nucleotide #: NM_000969.5
• UniProt Accession #: P46777; Q32LZ3; Q53HH6; Q9H3F4
• Molecular Weight: 34 KD
• NCBI Official Full Name: 60S ribosomal protein L5
• NCBI Official Synonym Full Names: ribosomal protein L5
• NCBI Official Symbol: RPL5
• NCBI Official Synonym Symbols: L5; uL18; MSTP030; PPP1R135
• NCBI Protein Information: 60S ribosomal protein L5
• UniProt Protein Name: 60S ribosomal protein L5
• Protein Family: 50S ribosomal protein
• UniProt Gene Name: RPL5

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of four RNA species and approximately 80 structurally distinct proteins. This gene encodes a member of the L18P family of ribosomal proteins and component of the 60S subunit. The encoded protein binds 5S rRNA to form a stable complex called the 5S ribonucleoprotein particle (RNP), which is necessary for the transport of nonribosome-associated cytoplasmic 5S rRNA to the nucleolus for assembly into ribosomes. The encoded protein may also function to inhibit tumorigenesis through the activation of downstream tumor suppressors and the downregulation of oncoprotein expression. Mutations in this gene have been identified in patients with Diamond-Blackfan Anemia (DBA). This gene is co-transcribed with the small nucleolar RNA gene U21, which is located in its fifth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed throughout the genome. [provided by RefSeq, Mar 2017]

Uniprot Description

Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. As part of the 5S RNP/5S ribonucleoprotein particle it is an essential component of the LSU, required for its formation and the maturation of rRNAs (PubMed:12962325, PubMed:19061985, PubMed:24120868, PubMed:23636399). It also couples ribosome biogenesis to p53/TP53 activation. As part of the 5S RNP it accumulates in the nucleoplasm and inhibits MDM2, when ribosome biogenesis is perturbed, mediating the stabilization and the activation of TP53 (PubMed:24120868).