Caveolin-1 Antibody | anti-CAV1 antibody

Caveolin-1 (Phospho-Tyr14) Antibody

Cat. Num. AAA8510093

• Gene Names: CAV1; CGL3; PPH3; BSCL3; LCCNS; VIP21; MSTP085
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
• Synonyms: Caveolin-1; Caveolin-1 (Phospho-Tyr14) Antibody; anti-CAV1 antibody

• Reactivity: Human, Mouse, Rat
• Specificity: Caveolin-1(Phospho-Tyr14) Antibody detects endogenous levels of Caveolin-1 only when phosphorylated at Tyrosine 14.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 178

• Applicable Applications for anti-CAV1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; ELISA: 1:20000
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human Caveolin-1 around the phosphorylation site of Tyrosine 14(H-L-YP-T-V).
• Preparation and Storage: Store at-20 degree C for 1 year

Western Blot (WB)

(Western blot analysis of the extracts from NIH/3T3 cells untreated or treated with H2O2 using Caveolin-1(Ab-14) Antibody and Caveolin-1(Phospho-Tyr14) Antibody: Line1: Using Caveolin-1(Ab-14) Antibody; Line2: Using Caveolin-1(Ab-14) Antibody preincubated with synthesized peptide; Line3 & Line4: Using Caveolin-1(Phospho-Tyr14) Antibody.)

References

Zhang Y, et al. (2005) Mol Cell Proteomics. 4(9): 1240-1250. Labrecque L, et al. (2004) J Biol Chem. 279(50): 52132-52140. Fielding PE, et al. (2004) Biochemistry. 43(9): 2578-2586. Maggi D, et al. (2002) Biochem Biophys Res Commun. 295(5): 1085-1089.

NCBI and Uniprot Product Information

• NCBI GI #: 15451856
• NCBI GeneID: 857
• NCBI Accession #: NP_001744.2
• NCBI GenBank Nucleotide #: NM_001753.4
• UniProt Accession #: Q03135; Q9UGP1; Q9UNG1; Q9UQH6
• Molecular Weight: 17,023 Da
• NCBI Official Full Name: caveolin-1 isoform alpha
• NCBI Official Synonym Full Names: caveolin 1
• NCBI Official Symbol: CAV1
• NCBI Official Synonym Symbols: CGL3; PPH3; BSCL3; LCCNS; VIP21; MSTP085
• NCBI Protein Information: caveolin-1
• UniProt Protein Name: Caveolin-1
• Protein Family: Caveolin
• UniProt Gene Name: CAV1
• UniProt Synonym Gene Names: CAV

NCBI Description

The scaffolding protein encoded by this gene is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 mitogen-activated kinase cascade. Caveolin 1 and caveolin 2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. Mutations in this gene have been associated with Berardinelli-Seip congenital lipodystrophy. Alternatively spliced transcripts encode alpha and beta isoforms of caveolin 1.[provided by RefSeq, Mar 2010]

Uniprot Description

May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway. Negatively regulates TGFB1-mediated activation of SMAD2/3 by mediating the internalization of TGFBR1 from membrane rafts leading to its subsequent degradation (PubMed:25893292). Mediates the recruitment of CAVIN proteins (CAVIN1/2/3/4) to the caveolae (PubMed:19262564).