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Testing Data (Peptide-+)

anti-Human SSB Antibody | anti-SSB antibody

SSB (Ab-366) Antibody

Gene Names
SSB; La; LARP3; La/SSB
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
SSB; SSB (Ab-366) Antibody; La antigen; La autoantigen; La ribonucleoprotein; Lupus La protein; SS-B; Sjogren syndrome type B antigen; anti-SSB antibody
Ordering
For Research Use Only!
Reactivity
Human
Specificity
SSB (Ab-366) antibody detects endogenous levels of total SSB protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
408
Applicable Applications for anti-SSB antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:40000
Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human SSB around the phosphorylation site of serine 366 (F-A-SP-D-D).
Preparation and Storage
Store at-20 degree C for 1 year.

Testing Data

(Peptide-+)

Testing Data (Peptide-+)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using SSB (Ab-366) antibody. Western blot analysis of extracts from 293 cells, using SSB (Ab-366) antibody.)

Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using SSB (Ab-366) antibody. Western blot analysis of extracts from 293 cells, using SSB (Ab-366) antibody.)
References
Fan H., Cell 88:707-715(1997). Fouraux M.A., RNA 8:1428-1443(2002). Olsen J.V., Cell 127:635-648(2006).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
46,837 Da
NCBI Official Full Name
lupus La protein
NCBI Official Synonym Full Names
Sjogren syndrome antigen B
NCBI Official Symbol
SSB
NCBI Official Synonym Symbols
La; LARP3; La/SSB
NCBI Protein Information
lupus La protein
UniProt Protein Name
Lupus La protein
Protein Family
UniProt Gene Name
SSB
UniProt Synonym Gene Names
SS-B

NCBI Description

The protein encoded by this gene is involved in diverse aspects of RNA metabolism, including binding and protecting poly(U) termini of nascent RNA polymerase III transcripts from exonuclease digestion, processing 5' and 3' ends of pre-tRNA precursors, acting as an RNA chaperone, and binding viral RNAs associated with hepatitis C virus. Autoantibodies reacting with this protein are found in the sera of patients with Sjogren syndrome and systemic lupus erythematosus. Alternative promoter usage results in two different transcript variants which encode the same protein. [provided by RefSeq, Jun 2014]

Uniprot Description

Binds to the 3' poly(U) terminus of nascent RNA polymerase III transcripts, protecting them from exonuclease digestion and facilitating their folding and maturation (PubMed:3192525, PubMed:2470590). In case of Coxsackievirus B3 infection, binds to the viral internal ribosome entry site (IRES) and stimulates the IRES-mediated translation (PubMed:12384597).

Research Articles on SSB

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Product Notes

The SSB ssb (Catalog #AAA8509795) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The SSB (Ab-366) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SSB can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000. Researchers should empirically determine the suitability of the SSB ssb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SSB, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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