CKMT2 Antibody | anti-CKMT2 antibody

CKMT2 Antibody

Cat. Num. AAA8509725

• Gene Names: CKMT2; SMTCK
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: CKMT2; CKMT2 Antibody; Creatine kinase; sarcomeric mitochondrial [Precursor]; EC 2.7.3.2; S-MtCK; Mib-CK; Basic-type mitochondrial creatine kinase; anti-CKMT2 antibody

• Reactivity: Human, Mouse, Rat
• Specificity: CKMT2 antibody detects endogenous levels of total CKMT2 protein.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 419

• Applicable Applications for anti-CKMT2 antibody: Western Blot (WB), Immunofluorescence (IF), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; IF: 1:100~1:500; ELISA: 1:40000
• Immunogen: The antiserum was produced against synthesized peptide derived from internal of human CKMT2.
• Preparation and Storage: Store at-20 degree C for 1 year.

Testing Data

Western Blot (WB)

(Peptide-+ Western blot analysis of extracts from A549 cells, using CKMT2 antibody. Immunofluorescence analysis of NIH/3T3 cells, using CKMT2 antibody.)

References

Haas R.C., J. Biol. Chem. 265:6921-6927(1990). The MGC Project Team, Genome Res. 14:2121-2127(2004). Haas R.C., J. Biol. Chem. 264:2890-2897(1989).

NCBI and Uniprot Product Information

• NCBI GI #: 153251984
• NCBI GeneID: 1160
• NCBI Accession #: NP_001093205.1
• NCBI GenBank Nucleotide #: NM_001099735.1
• UniProt Accession #: P17540; Q6ICS8; Q8N1E1
• Molecular Weight: 47,504 Da
• NCBI Official Full Name: creatine kinase S-type, mitochondrial
• NCBI Official Synonym Full Names: creatine kinase, mitochondrial 2
• NCBI Official Symbol: CKMT2
• NCBI Official Synonym Symbols: SMTCK
• NCBI Protein Information: creatine kinase S-type, mitochondrial
• UniProt Protein Name: Creatine kinase S-type, mitochondrial
• Protein Family: Creatine kinase
• UniProt Gene Name: CKMT2
• UniProt Synonym Gene Names: Mib-CK; S-MtCK

NCBI Description

Mitochondrial creatine kinase (MtCK) is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Sarcomeric mitochondrial creatine kinase has 80% homology with the coding exons of ubiquitous mitochondrial creatine kinase. This gene contains sequences homologous to several motifs that are shared among some nuclear genes encoding mitochondrial proteins and thus may be essential for the coordinated activation of these genes during mitochondrial biogenesis. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.