anti-Human, Mouse Cyclin E2 Antibody | anti-CCNE2 antibody

Cyclin E2 (Phospho-Thr392) Antibody

Cat. Num. AAA8509630

• Gene Names: CCNE2; CYCE2
• Reactivity: Human, Mouse
• Applications: Immunofluorescence, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Synonyms: Cyclin E2; Cyclin E2 (Phospho-Thr392) Antibody; CGE2; CYCE2; G1/S-Specific cyclin E2; anti-CCNE2 antibody

• Reactivity: Human, Mouse
• Specificity: Cyclin E2 (Phospho-Thr392) antibody detects endogenous levels of Cyclin E2 only when phosphorylated at threonine 392.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 404

• Applicable Applications for anti-CCNE2 antibody: Immunofluorescence (IF), ELISA (EIA)
• Application Notes: IF: 1:100-1:500; ELISA: 1:10000
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human Cyclin E2 around the phosphorylation site of threonine 392 (I-M-TP-P-P).
• All Sites: Human: Thr392; Mouse: Thr392
• Preparation and Storage: Store at-20 degree C for 1 year.

Testing Data

(P-peptide-+)

Immunofluorescence (IF)

(Immunofluorescence analysis of NIH/3T3 cells, using Cyclin E2 (Phospho-Thr392) antibody. Cyclin E2 (Phospho-Thr392) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)

References

Lisa K. Naeger. Cell Growth Differ., Jun 1999; 10: 413. Francisco Noya. J. Virol., Jul 2001; 75: 6121-6134. Suparna Mazumder. Mol. Cell. Biol., May 2007; 27: 3511-3520.

NCBI and Uniprot Product Information

• NCBI GI #: 17318565
• NCBI GeneID: 9134
• NCBI Accession #: NP_477097.1
• NCBI GenBank Nucleotide #: NM_057749.2
• UniProt Accession #: O96020; O95439
• Molecular Weight: 41,465 Da
• NCBI Official Full Name: G1/S-specific cyclin-E2
• NCBI Official Synonym Full Names: cyclin E2
• NCBI Official Symbol: CCNE2
• NCBI Official Synonym Symbols: CYCE2
• NCBI Protein Information: G1/S-specific cyclin-E2
• UniProt Protein Name: G1/S-specific cyclin-E2
• Protein Family: G1/S-specific cyclin
• UniProt Gene Name: CCNE2

NCBI Description

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2. This cyclin has been shown to specifically interact with CIP/KIP family of CDK inhibitors, and plays a role in cell cycle G1/S transition. The expression of this gene peaks at the G1-S phase and exhibits a pattern of tissue specificity distinct from that of cyclin E1. A significantly increased expression level of this gene was observed in tumor-derived cells. [provided by RefSeq, Jul 2008]

Uniprot Description

Essential for the control of the cell cycle at the late G1 and early S phase.