anti-Human CD50/ICAM-3 Antibody | anti-CD50/ICAM-3 antibody

CD50/ICAM-3 (Ab-518) Antibody

Cat. Num. AAA8509625

• Gene Names: ICAM3; CD50; CDW50; ICAM-R
• Reactivity: Human
• Applications: Western Blot, Immunofluorescence, ELISA
• Purity: Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: CD50/ICAM-3; CD50/ICAM-3 (Ab-518) Antibody; CD50 antigen; CDw50; ICA3; ICAM-R; ICAM3; Intercellular adhesion molecule-3 precursor; anti-CD50/ICAM-3 antibody

• Reactivity: Human
• Specificity: CD50/ICAM-3 (Ab-518) antibody detects endogenous levels of total CD50/ICAM-3 protein.
• Purity/Purification: Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 547

• Applicable Applications for anti-CD50/ICAM-3 antibody: Western Blot (WB), Immunofluorescence (IF), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; IF: 1:100~1:500; ELISA: 1:5000
• Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human CD50/ICAM-3 around the phosphorylation site of serine 518 (S-G-SP-Y-H).
• Preparation and Storage: Store at-20 degree C for 1 year.

Testing Data

Western Blot (WB)

(Peptide-+ Western blot analysis of extracts from NIH/3T3 cells, treated with TNF-a (20ng/ml, 5mins), using CD50/ICAM-3 (Ab-518) antibody. Immunofluorescence analysis of A549 cells, using CD50/ICAM-3 (Ab-518) antibody.)

References

Fawcett J., Nature 360:481-484(1992). de Fougerolles A.R., J. Exp. Med. 177:1187-1192(1993). Funatsu O., Eur. J. Biochem. 268:1020-1029(2001). Liu T., J. Proteome Res. 4:2070-2080(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 167466207
• NCBI GeneID: 3385
• NCBI Accession #: NP_002153.2
• NCBI GenBank Nucleotide #: NM_002162.4
• UniProt Accession #: P32942; Q6PD68
• Molecular Weight: 59,541 Da
• NCBI Official Full Name: intercellular adhesion molecule 3 isoform 1
• NCBI Official Synonym Full Names: intercellular adhesion molecule 3
• NCBI Official Symbol: ICAM3
• NCBI Official Synonym Symbols: CD50; CDW50; ICAM-R
• NCBI Protein Information: intercellular adhesion molecule 3
• UniProt Protein Name: Intercellular adhesion molecule 3
• UniProt Gene Name: ICAM3
• UniProt Synonym Gene Names: ICAM-3

NCBI Description

The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Feb 2016]

Uniprot Description

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2) (PubMed:1448173). ICAM3 is also a ligand for integrin alpha-D/beta-2. In association with integrin alpha-L/beta-2, contributes to apoptotic neutrophil phagocytosis by macrophages (PubMed:23775590).