anti-Human, Mouse PR Antibody | anti-PR antibody

PR (Phospho-Ser294) Antibody

Cat. Num. AAA8509504

• Gene Names: PGR; PR; NR3C3
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunofluorescence, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Synonyms: PR; PR (Phospho-Ser294) Antibody; NR3C3; PGR; PRGR; Progesterone receptor; anti-PR antibody

• Reactivity: Human, Mouse
• Specificity: PR (Phospho-Ser294) antibody detects endogenous levels of PR only when phosphorylated at serine 294.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 933

• Applicable Applications for anti-PR antibody: Western Blot (WB), Immunofluorescence (IF), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; IF: 1:100~1:500; ELISA: 1:1000
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human PR around the phosphorylation site of serine 294 (G-R-SP-P-L).
• All Sites: Human: Ser294; Mouse: Ser294
• Preparation and Storage: Store at-20 degree C for 1 year.

Testing Data

Western Blot (WB)

(P-peptide-+ Western blot analysis of extracts from Jurkat cells, treated with Etoposide (25uM, 60mins), using PR (Phospho-Ser294) antibody. Immunofluorescence analysis of HeLa cells, using PR (Phospho-Ser294) antibody.)

References

Kastner P., EMBO J. 9:1603-1614(1990). Knotts T.A., J. Biol. Chem. 276:8475-8483(2001). Hsiao P.W., Mol. Cell. Biol. 23:6210-6220(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 110611914
• NCBI GeneID: 5241
• NCBI Accession #: NP_000917.3
• NCBI GenBank Nucleotide #: NM_000926.4
• UniProt Accession #: P06401; Q8TDS3; Q9UPF7; A7LQ08; A7X8B0; B4E3T0
• Molecular Weight: 87,747 Da
• NCBI Official Full Name: progesterone receptor isoform B
• NCBI Official Synonym Full Names: progesterone receptor
• NCBI Official Symbol: PGR
• NCBI Official Synonym Symbols: PR; NR3C3
• NCBI Protein Information: progesterone receptor
• UniProt Protein Name: Progesterone receptor
• Protein Family: Prestin
• UniProt Gene Name: PGR
• UniProt Synonym Gene Names: NR3C3; PR

NCBI Description

This gene encodes a member of the steroid receptor superfamily. The encoded protein mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. This gene uses two distinct promotors and translation start sites in the first exon to produce several transcript variants, both protein coding and non-protein coding. Two of the isoforms (A and B) are identical except for an additional 165 amino acids found in the N-terminus of isoform B and mediate their own response genes and physiologic effects with little overlap. [provided by RefSeq, Sep 2015]

Uniprot Description

The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Depending on the isoform, progesterone receptor functions as transcriptional activator or repressor.