anti-Human, Mouse NFAT3 Antibody | anti-NFAT3 antibody

NFAT3 (Ab-676) Antibody

Cat. Num. AAA8509480

• Gene Names: NFATC4; NFAT3; NF-AT3; NF-ATC4
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: NFAT3; NFAT3 (Ab-676) Antibody; NF-AT3; NF-ATc4; NFAC4; NFATC4; NFC4; Nuclear factor of activated T-cells; cytoplasmic 4; T cell transcription factor NFAT3; Transcription complex subunit NF-ATc4; anti-NFAT3 antibody

• Reactivity: Human, Mouse
• Specificity: NFAT3 (Ab-676) antibody detects endogenous levels of total NFAT3 protein.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 964

• Applicable Applications for anti-NFAT3 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:100; IF: 1:100~1:500; ELISA: 1:5000
• Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human NFAT3 around the phosphorylation site of serine 676(K-R-SP-P-T).
• Preparation and Storage: Store at-20 degree C for 1 year.

Testing Data

(Peptide-+)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using NFAT3 (Ab-676) antibody. Western blot analysis of extracts from 293 cells, using NFAT3 (Ab-676) antibody.)

References

Hoey T., Immunity 2:461-472(1995). Crabtree G.R., Cell 96:611-614(1999). Olsen J.V., Cell 127:635-648(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 209862845
• NCBI GeneID: 4776
• NCBI Accession #: NP_001129494.1
• NCBI GenBank Nucleotide #: NM_001136022.2
• UniProt Accession #: Q14934; B4DDG5; B4DY55; B5B2U7; B5B2U8; B5B2U9; B5B2V0; B5B2V1; B5B2V2; B5B2V3; B5B2V4; B5B2V5
• Molecular Weight: 8,950 Da
• NCBI Official Full Name: nuclear factor of activated T-cells, cytoplasmic 4 isoform 1
• NCBI Official Synonym Full Names: nuclear factor of activated T cells 4
• NCBI Official Symbol: NFATC4
• NCBI Official Synonym Symbols: NFAT3; NF-AT3; NF-ATC4
• NCBI Protein Information: nuclear factor of activated T-cells, cytoplasmic 4
• UniProt Protein Name: Nuclear factor of activated T-cells, cytoplasmic 4
• UniProt Gene Name: NFATC4
• UniProt Synonym Gene Names: NFAT3; NF-ATc4; NFATc4; NF-AT3

NCBI Description

This gene encodes a member of the nuclear factor of activated T cells (NFAT) protein family. The encoded protein is part of a DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor stimulation and an inducible nuclear component. NFAT proteins are activated by the calmodulin-dependent phosphatase, calcineurin. The encoded protein plays a role in the inducible expression of cytokine genes in T cells, especially in the induction of interleukin-2 and interleukin-4. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 and IL-4. Transcriptionally repressed by estrogen receptors; this inhibition is further enhanced by estrogen. Increases the transcriptional activity of PPARG and has a direct role in adipocyte differentiation. May play an important role in myotube differentiation. May play a critical role in cardiac development and hypertrophy. May play a role in deafferentation-induced apoptosis of sensory neurons.