anti-Human, Mouse HSP105 Antibody | anti-HSP105 antibody

HSP105 Antibody

Cat. Num. AAA8509378

• Gene Names: HSPH1; HSP105; HSP105A; HSP105B; NY-CO-25
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: HSP105; HSP105 Antibody; heat shock 105kDa/110kDa protein 1; anti-HSP105 antibody

• Reactivity: Human, Mouse
• Specificity: HSP105 antibody detects endogenous levels of total HSP105 protein.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 814

• Applicable Applications for anti-HSP105 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:100; IF: 1:100~1:500; ELISA: 1:10000
• Immunogen: The antiserum was produced against synthesized peptide derived from human HSP105.
• Preparation and Storage: Store at-20 degree C for 1 year

Immunohistochemistry (IHC)

(Peptide-+ Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using HSP105 antibody.)

Western Blot (WB)

(Western blot analysis of extracts from HeLa cells, using HSP105 antibody.)

Immunofluorescence (IF)

(Peptide-+ Immunofluorescence analysis of HeLa cells, using HSP105 antibody.)

References

Kunihiko Yasuda, J. Biol. Chem., Dec 1995; 270: 29718. Nobuyuki Yamagishi, Eur. J. Biochem., Aug 2002; 269: 4143. Nobuyuki Yamagishi, J. Biol. Chem., Oct 2004; 279: 41727-41733. Nobuyuki Yamagishi, J. Biochem. (Tokyo), Aug 2002; 132: 271-278.

NCBI and Uniprot Product Information

• NCBI GI #: 557357685
• NCBI GeneID: 10808
• NCBI Accession #: NP_001273432.1
• NCBI GenBank Nucleotide #: NM_001286503.1
• UniProt Accession #: Q92598; O95739; Q5TBM6; Q5TBM7; Q5TBM8; Q9UPC4; B4DYH1
• Molecular Weight: 97,460 Da
• NCBI Official Full Name: heat shock protein 105 kDa isoform 2
• NCBI Official Synonym Full Names: heat shock protein family H (Hsp110) member 1
• NCBI Official Symbol: HSPH1
• NCBI Official Synonym Symbols: HSP105; HSP105A; HSP105B; NY-CO-25
• NCBI Protein Information: heat shock protein 105 kDa
• UniProt Protein Name: Heat shock protein 105 kDa
• UniProt Gene Name: HSPH1
• UniProt Synonym Gene Names: HSP105; HSP110; KIAA0201

NCBI Description

This gene encodes a member of the heat shock protein 70 family of proteins. The encoded protein functions as a nucleotide exchange factor for the molecular chaperone heat shock cognate 71 kDa protein (Hsc70). In addition, this protein plays a distinct but related role as a holdase that inhibits the aggregation of misfolded proteins, including the cystic fibrosis transmembrane conductance regulator (CFTR) protein. Elevated expression of this protein has been observed in numerous human cancers. [provided by RefSeq, Mar 2017]

Uniprot Description

Acts as a nucleotide-exchange factor (NEF) for chaperone proteins HSPA1A and HSPA1B, promoting the release of ADP from HSPA1A/B thereby triggering client/substrate protein release (PubMed:24318877). Prevents the aggregation of denatured proteins in cells under severe stress, on which the ATP levels decrease markedly. Inhibits HSPA8/HSC70 ATPase and chaperone activities ().