COT Antibody | anti-COT antibody

COT (Phospho-Thr290) Antibody

Cat. Num. AAA8509337

• Gene Names: MAP3K8; COT; EST; ESTF; TPL2; AURA2; MEKK8; Tpl-2; c-COT
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Synonyms: COT; COT (Phospho-Thr290) Antibody; EC 1.14.16.2; TH isoform 3; TH isoform a; TH-4; TY3H; TYH; Tyrosine 3-hydroxylase; Tyrosine 5-monooxygenase; tyrosine hydroxylase; anti-COT antibody

• Reactivity: Human, Mouse, Rat
• Specificity: COT (phospho-Thr290) antibody detects endogenous levels of COT only when phosphorylated at threonine 290.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 467

• Applicable Applications for anti-COT antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:100; IF: 1:100~1:500; ELISA: 1:40000
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human COT around the phosphorylation site of threonine 290 (R-G-TP-E-I).
• All Sites: Human: Thr290; Mouse: Thr290; Rat: Thr290
• Preparation and Storage: Store at-20 degree C for 1 year

Immunohistochemistry (IHC)

(P-peptide-+ Immunohistochemical analysis of paraffin-embedded human brain tissue using COT (phospho-Thr290) antibody.)

Western Blot (WB)

(Western blot analysis of extracts from 293 cells, treated with UV (15mins), using COT (phospho-Thr290) antibody.)

Immunofluorescence (IF)

(P-peptide-+ Immunofluorescence analysis of HuvEc cells, using COT (phospho-Thr290) antibody (#11364).)

References

Jahan Ara, PNAS, Jun 1998; 95: 7659. E Carafoli, Crit. Rev. Biochem. Mol. Biol., Apr 2001; 36: 107. P. William Conrad, J. Biol. Chem., Nov 1999; 274: 33709. Y. Katayama, J Appl Physiol, Nov 1994; 77: 2086.

NCBI and Uniprot Product Information

• NCBI GI #: 346644795
• NCBI GeneID: 1326
• NCBI Accession #: NP_001231063.1
• NCBI GenBank Nucleotide #: NM_001244134.1
• UniProt Accession #: P41279; Q14275; Q5T855; Q9HC81; A8K2Q5; D3DRX1
• Molecular Weight: 49,620 Da
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase 8
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase 8
• NCBI Official Symbol: MAP3K8
• NCBI Official Synonym Symbols: COT; EST; ESTF; TPL2; AURA2; MEKK8; Tpl-2; c-COT
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase 8
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase 8
• UniProt Gene Name: MAP3K8
• UniProt Synonym Gene Names: COT; ESTF; TPL-2

NCBI Description

This gene is an oncogene that encodes a member of the serine/threonine protein kinase family. The encoded protein localizes to the cytoplasm and can activate both the MAP kinase and JNK kinase pathways. This protein was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. This protein was also found to promote the production of TNF-alpha and IL-2 during T lymphocyte activation. This gene may also utilize a downstream in-frame translation start codon, and thus produce an isoform containing a shorter N-terminus. The shorter isoform has been shown to display weaker transforming activity. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2011]

Uniprot Description

Required for lipopolysaccharide (LPS)-induced, TLR4-mediated activation of the MAPK/ERK pathway in macrophages, thus being critical for production of the proinflammatory cytokine TNF-alpha (TNF) during immune responses. Involved in the regulation of T-helper cell differentiation and IFNG expression in T-cells. Involved in mediating host resistance to bacterial infection through negative regulation of type I interferon (IFN) production. In vitro, activates MAPK/ERK pathway in response to IL1 in an IRAK1-independent manner, leading to up-regulation of IL8 and CCL4. Transduces CD40 and TNFRSF1A signals that activate ERK in B-cells and macrophages, and thus may play a role in the regulation of immunoglobulin production. May also play a role in the transduction of TNF signals that activate JNK and NF-kappa-B in some cell types. In adipocytes, activates MAPK/ERK pathway in an IKBKB-dependent manner in response to IL1B and TNF, but not insulin, leading to induction of lipolysis. Plays a role in the cell cycle. Isoform 1 shows some transforming activity, although it is much weaker than that of the activated oncogenic variant.