anti-Human, Mouse Synaptotagmin Antibody | anti-SYT antibody

Synaptotagmin (Phospho-Ser309) Antibody

Cat. Num. AAA8509323

• Gene Names: SYT1; P65; SYT; SVP65
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Synonyms: Synaptotagmin; Synaptotagmin (Phospho-Ser309) Antibody; p65; synaptotagmin I; anti-SYT antibody

• Reactivity: Human, Mouse
• Specificity: Synaptotagmin (phospho-Ser309) antibody detects endogenous levels of Synaptotagmin only when phosphorylated at serine 309.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 422

• Applicable Applications for anti-SYT antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: WB: 1:500~1000; IHC: 1:50~1:100; ELISA: 1:10000
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human Synaptotagmin around the phosphorylation site of serine 309 (G-L-SP-D-P).
• All Sites: Human: Ser309; Mouse: Ser308
• Preparation and Storage: Store at-20 degree C for 1 year

Immunohistochemistry (IHC)

(P-peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Synaptotagmin (phospho-Ser309) antibody.)

Western Blot (WB)

(Western blot analysis of extracts from 293 cells treated with Sobital (0.4M, 30mins), using Synaptotagmin (phospho-Ser309) antibody.)

References

E Carafoli, Crit. Rev. Biochem. Mol. Biol., Apr 2001; 36: 107. Gry Houeland, J Neurophysiol, Jan 2007; 97: 134-143. Christelle Carteron, J. Cell Sci., Mar 2006; 119: 898-909. Vladimir I. Slepnev, Science, Aug 1998; 281: 821.

NCBI and Uniprot Product Information

• NCBI GI #: 209447070
• NCBI GeneID: 6857
• NCBI Accession #: NP_001129277.1
• NCBI GenBank Nucleotide #: NM_001135805.1
• UniProt Accession #: P21579; Q6AI31
• Molecular Weight: 47,573 Da
• NCBI Official Full Name: synaptotagmin-1 isoform 1
• NCBI Official Synonym Full Names: synaptotagmin 1
• NCBI Official Symbol: SYT1
• NCBI Official Synonym Symbols: P65; SYT; SVP65
• NCBI Protein Information: synaptotagmin-1
• UniProt Protein Name: Synaptotagmin-1
• Protein Family: Synaptotagmin
• UniProt Gene Name: SYT1
• UniProt Synonym Gene Names: SVP65; SYT; SytI

NCBI Description

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin-1 participates in triggering neurotransmitter release at the synapse (Fernandez-Chacon et al., 2001 [PubMed 11242035]).[supplied by OMIM, Jul 2010]

Uniprot Description

May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca2+-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca2+-independent manner; these are neurexins, syntaxin and AP2. Plays a role in dendrite formation by melanocytes (PubMed:23999003).