Rabbit anti-Human, Mouse SETDB1 Antibody | anti-SETDB1 antibody

SETDB1 Antibody

Cat. Num. AAA8503004

• Gene Names: SETDB1; ESET; KG1T; KMT1E; TDRD21; H3-K9-HMTase4
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: Affinity purification
• Synonyms: SETDB1; SETDB1 Antibody; ESET; H3-K9-HMTase4; KG1T; KIAA0067; KMT1E; anti-SETDB1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Isotype: IgG
• Purity/Purification: Affinity purification
• Sequence Length: 1291

• Applicable Applications for anti-SETDB1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:100; IP: 1:50-1:100
• Immunogen: Fusion protein of human SETDB1
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.

Related Product Information for anti-SETDB1 antibody

The Erg-associated protein with SET domain (ESET), also known as SET-domain, bifurcated 1 (SETDB1) protein, is a member of a family of histone lysine methyltransferases, each of which contains a conserved catalytic SET domain originally identified in Drosophila Su[var]3-9, Enhancer of zeste, and Trithorax proteins (1). ESET also contains tudor and methyl-CpG-binding domains, which may coordinate binding to methylated histones and methylated DNA, respectively (1). ESET methylates histone H3 Lys9, creating a transcriptionally repressive mark that facilitates gene silencing (1-3). However, unlike SUV39H histone H3 Lys9 methyltransferases, which function mainly in heterochromatin regions such as pericentric heterochromatin, ESET functions mainly in euchromatic regions to repress gene promoters (3). ESET interacts with a variety of proteins, including transcription factors (ERG), histone deacetylases (HDAC1/2), DNA methyltransferases (DNMT3A/B) and transcriptional co-repressors (mSin3A/B, MBD1, KAP-1, the ATFa-associated modulator mAM) (1-6). mAM forms a complex with ESET, stimulating its methyltransferase activity, specifically the conversion of di-methyl to tri-methyl histone H3 Lys9 (2). MBD1 recruits ESET to the CAF-1 complex to facilitate methylation of histone H3 Lys9 during replication-coupled chromatin assembly in S phase (5). DNMT3A recruits ESET to silenced promoters in cancer cells (7). ESET may play a role in the pathogenesis of Huntington's disease, since levels of ESET protein and tri-methyl histone H3 Lys9 are both increased in diseased brains (8).

Product Categories/Family for anti-SETDB1 antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

1. Yang, L. et al. (2002) Oncogene 21, 148-152. 2. Wang, H. et al. (2003) Mol. Cell 12, 475-487. 3. Schultz, D.C. et al. (2002) Genes Dev. 16, 919-932. 4. Yang, L. et al. (2003) Biochem. J. 369, 651-657. 5. Sarraf, S.A. and Stancheva, I. (2004) Mol. Cell 15, 595-605. 6. Ichimura, T. et al. (2005) J. Biol. Chem. 280, 13928-13935. 7. Li, H. et al. (2006) J. Biol. Chem. 281, 19489-19500. 8. Ryu, H. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 19176-19181.

NCBI and Uniprot Product Information

• NCBI GI #: 224177467
• NCBI GeneID: 9869
• NCBI Accession #: NP_001138887.1
• NCBI GenBank Nucleotide #: NM_001145415.1
• UniProt Accession #: Q15047; Q5SZD8; Q5SZD9; Q5SZE0; Q5SZE7; Q96GM9; A6NEW2
• Molecular Weight: 143kDa
• NCBI Official Full Name: histone-lysine N-methyltransferase SETDB1 isoform 1
• NCBI Official Synonym Full Names: SET domain bifurcated 1
• NCBI Official Symbol: SETDB1
• NCBI Official Synonym Symbols: ESET; KG1T; KMT1E; TDRD21; H3-K9-HMTase4
• NCBI Protein Information: histone-lysine N-methyltransferase SETDB1
• UniProt Protein Name: Histone-lysine N-methyltransferase SETDB1
• Protein Family: Histone-lysine N-methyltransferase
• UniProt Gene Name: SETDB1
• UniProt Synonym Gene Names: KIAA0067; KMT1E; ESET; H3-K9-HMTase 4

NCBI Description

This gene encodes a histone methyltransferase which regulates histone methylation, gene silencing, and transcriptional repression. This gene has been identified as a target for treatment in Huntington Disease, given that gene silencing and transcription dysfunction likely play a role in the disease pathogenesis. Alternatively spliced transcript variants of this gene have been described.[provided by RefSeq, Jun 2011]

Uniprot Description

Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 'Lys-9' trimethylation is coordinated with DNA methylation. Probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed:24623306). Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed:24623306). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed:24623306). The SETDB1-TRIM28-ZNF274 complex may play a role in recruiting ATRX to the 3'-exons of zinc-finger coding genes with atypical chromatin signatures to establish or maintain/protect H3K9me3 at these transcriptionally active regions (PubMed:27029610).