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Testing Data (Figure 1. Western blot analysis using MYST1 mouse mAb against Hela (1), HepG2 (2) and SMMC-7721 (3) cell lysate.)

Mouse anti-Human MYST1 Antibody | anti-MYST1 antibody

MYST1 Antibody

Gene Names
KAT8; MOF; hMOF; MYST1; ZC2HC8
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Synonyms
MYST1; MYST1 Antibody; MOF; KAT8; hMOF; anti-MYST1 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Isotype
IgG2b
Clone Number
8C4C4
Form/Format
Ascitic fluid containing 0.03% sodium azide.
Sequence Length
458
Applicable Applications for anti-MYST1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:200-1:1000
IF: 1:200-1:1000
ELISA: 1:10000
Immunogen
Purified recombinant fragment of human MYST1 expressed in E. Coli.
Preparation and Storage
Store at 4 degree C, for long term storage, store at-20 degree C

Testing Data

(Figure 1. Western blot analysis using MYST1 mouse mAb against Hela (1), HepG2 (2) and SMMC-7721 (3) cell lysate.)

Testing Data (Figure 1. Western blot analysis using MYST1 mouse mAb against Hela (1), HepG2 (2) and SMMC-7721 (3) cell lysate.)

Testing Data #2

(Figure 3. Confocal immunofluorescence analysis of Eca 109 cells using MOF/MYST1 mouse mAb (green), showing nuclear localization.)

Testing Data #2 (Figure 3. Confocal immunofluorescence analysis of Eca 109 cells using MOF/MYST1 mouse mAb (green), showing nuclear localization.)

Testing Data #3

(Figure 2. Immunohistochemical analysis of paraffin-embedded human esophageal squamous cell carcinoma (A), normal esophagus epithelium (B), rectum adenocarcinoma (C), lung squamous cell carcinoma (D), breast infiltrating carcinoma (E), and breast infiltrating carcinoma (F) tissues, showing nuclear localization using MOF/MYST1 mouse mAb with DAB staining.)

Testing Data #3 (Figure 2. Immunohistochemical analysis of paraffin-embedded human esophageal squamous cell carcinoma (A), normal esophagus epithelium (B), rectum adenocarcinoma (C), lung squamous cell carcinoma (D), breast infiltrating carcinoma (E), and breast infiltrating carcinoma (F) tissues, showing nuclear localization using MOF/MYST1 mouse mAb with DAB staining.)
Related Product Information for anti-MYST1 antibody
MYST1 (MYST histone acetyltransferase 1, MOF) belongs to the MYST family of histone acetyltransferases, which are employed in the cell to bring about transcriptional regulation. The MYST family includes MYST1, is named for the founding members MOZ, yeast YBF2 and SAS2, and TIP60. All members of this family contain a MYST region of about 240 amino acids with a canonical acetyl-CoA-binding site and a C2HC-type zinc finger motif. Most MYST proteins also have a chromodomain involved in protein-protein interactions and targeting transcriptional regulators to chromatin. Although MOF is expressed in both males and females, it associates with the X chromosome only in males. MOF contains a zinc-finger domain that is used to contact the globular part of the nucleosome and histone H4.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
53Kd
NCBI Official Full Name
histone acetyltransferase KAT8 isoform 1
NCBI Official Synonym Full Names
lysine acetyltransferase 8
NCBI Official Symbol
KAT8
NCBI Official Synonym Symbols
MOF; hMOF; MYST1; ZC2HC8
NCBI Protein Information
histone acetyltransferase KAT8
UniProt Protein Name
Histone acetyltransferase KAT8
UniProt Gene Name
KAT8
UniProt Synonym Gene Names
MOF; MYST1; MYST-1; hMOF

NCBI Description

This gene encodes a member of the MYST histone acetylase protein family. The encoded protein has a characteristic MYST domain containing an acetyl-CoA-binding site, a chromodomain typical of proteins which bind histones, and a C2HC-type zinc finger. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]

Uniprot Description

Histone acetyltransferase which may be involved in transcriptional activation. May influence the function of ATM. As part of the MSL complex it is involved in acetylation of nucleosomal histone H4 producing specifically H4K16ac. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. That activity is less specific than the one of the MSL complex. Can also acetylate TP53/p53 at 'Lys-120'.

Research Articles on MYST1

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Product Notes

The MYST1 kat8 (Catalog #AAA850250) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MYST1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MYST1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:200-1:1000 IF: 1:200-1:1000 ELISA: 1:10000. Researchers should empirically determine the suitability of the MYST1 kat8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MYST1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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