Rabbit KEAP1 Antibody | anti-KEAP1 antibody

KEAP1 Antibody

Cat. Num. AAA8501631

• Gene Names: KEAP1; INrf2; KLHL19
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity purification
• Synonyms: KEAP1; KEAP1 Antibody; INrf2; KIAA0132; KLHL19; MGC10630; MGC1114; MGC20887; MGC4407; MGC9454; anti-KEAP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Purity/Purification: Affinity purification
• Sequence Length: 624

• Applicable Applications for anti-KEAP1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: Recombinant protein of human KEAP1
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.

Testing Data

(Western blot analysis of extracts of HepG2cell lines, using KEAP1 antibody.)

Related Product Information for anti-KEAP1 antibody

The nuclear factor-like 2 (NRF2) transcriptional activator binds antioxidant response elements (ARE) of target gene promoter regions to regulate expression of oxidative stress response genes. Under basal conditions, the NRF2 inhibitor INrf2 (also called KEAP1) binds and retains NRF2 in the cytoplasm where it can be targeted for ubiquitin-mediated degradation (1). Small amounts of constitutive nuclear NRF2 maintains cellular homeostasis through regulation of basal expression of antioxidant response genes. Following oxidative or electrophilic stress, KEAP1 releases NRF2, thereby allowing the activator to translocate to the nucleus and bind to ARE-containing genes (2). The coordinated action of NRF2 and other transcription factors mediates the response to oxidative stress (3). Altered expression of NRF2 is associated with chronic obstructive pulmonary disease (COPD) (4). NRF2 activity in lung cancer cell lines directly correlates with cell proliferation rates, and inhibition of NRF2 expression by siRNA enhances anti-cancer drug-induced apoptosis (5). The NRF2 repressor KEAP1 contains an amino terminal BTB/POZ domain and a carboxyl terminal KELCH domain (6, 7). The KELCH domain is required for interacting with NRF2 and the BTB/POZ domain functions in binding Cul3 E3 ubiquitin ligase (8-10). Under normal conditions, the complex leads to the cytoplasmic sequestration and ubiquitin-mediated proteasomal degradation of NRF2. Electrophilic modification of KEAP1 leads to disassociation of the NRF2/KEAP1 complex. KEAP1 also targets the down regulation of NF-kappaB activity by targeting IKKbeta degradation (11).

Product Categories/Family for anti-KEAP1 antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

1. Cullinan, S.B. et al. (2004) Mol Cell Biol 24, 8477-86. 2. Nguyen, T. et al. (2005) J BiolChem 280, 32485-92. 3. Jaiswal, A.K. (2004) Free RadicBiol Med 36, 1199-207. 4. Suzuki, M. et al. (2008) Am J Respir Cell MolBiol 39, 673-82. 5. Homma, S. et al. (2009) Clin Cancer Res 15, 3423-32. 6. Itoh, K. et al. (1999) Genes Dev 13, 76-86. 7. Dhakshinamoorthy, S. and Jaiswal, A.K. (2001) Oncogene 20, 3906-17. 8. Furukawa, M. and Xiong, Y. (2005) Mol Cell Biol 25, 162-71. 9. Zhang, D.D. et al. (2004) Mol Cell Biol 24, 10941-53. 10. Kobayashi, A. et al. (2004) Mol Cell Biol 24, 7130-9. 11. Lee, D.F. et al. (2009) Mol Cell 36, 131-40.

NCBI and Uniprot Product Information

• NCBI GI #: 22027642
• NCBI GeneID: 9817
• NCBI Accession #: NP_036421.2
• NCBI GenBank Nucleotide #: NM_012289.3
• UniProt Accession #: Q14145; Q6LEP0; Q8WTX1; Q9BPY9; B3KPD5
• Molecular Weight: 70kDa
• NCBI Official Full Name: kelch-like ECH-associated protein 1
• NCBI Official Synonym Full Names: kelch like ECH associated protein 1
• NCBI Official Symbol: KEAP1
• NCBI Official Synonym Symbols: INrf2; KLHL19
• NCBI Protein Information: kelch-like ECH-associated protein 1
• UniProt Protein Name: Kelch-like ECH-associated protein 1
• Protein Family: Kelch-like ECH-associated protein
• UniProt Gene Name: KEAP1
• UniProt Synonym Gene Names: INRF2; KIAA0132; KLHL19; INrf2

NCBI Description

This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Acts as a substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1 and targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. Retains NFE2L2/NRF2 and may also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome.