Testing Data
Rabbit AP-1 Polyclonal Antibody | anti-AP-1 antibody
Phospho-AP-1 (Ser63) Antibody
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Affinity-chromatography using epitope-specific immunogen
Synonyms
AP-1; Polyclonal Antibody; Phospho-AP-1 (Ser63) Antibody; JUN; Transcription factor AP-1; Activator protein 1; AP1; Proto-oncogene c-Jun; V-jun avian sarcoma virus 17 oncogene homolog; p39; anti-AP-1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity-chromatography using epitope-specific immunogen
Form/Format
Colorless liquid
In PBS containing 50% glycerol; 0.5% BSA and 0.02% sodium azide
In PBS containing 50% glycerol; 0.5% BSA and 0.02% sodium azide
Sequence Length
331
Applicable Applications for anti-AP-1 antibody
Western Blot (WB); ELISA (EIA); Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:100-1:300
ELISA: 1:20000
IHC: 1:100-1:300
ELISA: 1:20000
Immunogen
Synthesized peptide derived from human AP-1 around the phosphorylation site of S63 (10-90aa)
Preparation and Storage
Store at -20 degree C for 12 months.
The antibody solution should be gently mixed before use.
The antibody solution should be gently mixed before use.
Testing Data
Testing Data
Related Product Information for anti-AP-1 antibody
Polyclonal antibody to detect endogenous levels of AP-1 protein when phosphorylated at Ser63 in human; mouse and rat.
Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells. Binds to the USP28 promoter in colorectal cancer (CRC) cells
Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells. Binds to the USP28 promoter in colorectal cancer (CRC) cells
Product Categories/Family for anti-AP-1 antibody
NCBI and Uniprot Product Information
NCBI GeneID
Molecular Weight
35,676 Da
UniProt Protein Name
Transcription factor AP-1
Protein Family
UniProt Gene Name
JUN
UniProt Synonym Gene Names
AP1
Uniprot Description
Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306).
Similar Products
Product Notes
The AP-1 jun (Catalog #AAA846127) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-AP-1 (Ser63) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's AP-1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB); ELISA (EIA); Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:100-1:300 ELISA: 1:20000. Researchers should empirically determine the suitability of the AP-1 jun for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AP-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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