PDGFRb/CD140b Active Protein | PDGFRB active protein

Human CellExp PDGFRb / CD140b, human recombinant

Cat. Num. AAA845361

• Gene Names: PDGFRB; IMF1; IBGC4; JTK12; PDGFR; CD140B; PDGFR1; PDGFR-1
• Applications: SDS-Page
• Purity: >=95%
• Synonyms: PDGFRb/CD140b; Human CellExp PDGFRb / CD140b; human recombinant; PDGFRB; PDGFR-B; CD140B; CD-140B; JTK12; JTK-12; PDGF-R-beta; PDGFR; PDGFR1; PDGFR-1; PDGFR-beta; PDGFRB active protein

• Host: HEK293 cells
• Purity/Purification: >=95%
• Form/Format: Lyophilized from 0.22 mum filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.; Appearance: Lyophilized
• Sequence Length: 1106

• Applicable Applications for PDGFRB active protein: SDS-PAGE
• Solubility/Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 ug/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 month. For extended storage, it is recommended to store at -20 degree C.
• Endotoxin Level: <1 EU/mug by LAL method
• Biological Activity: Measured by its ability to inhibit the biological activity of PDGFBB using NR6R-3T3 mouse fibroblast cells. The ED50 for this effect is typically 3-8 mug/ml in the presence of 4 ng/ml rhPDGFBB.
• Results: Measured by its ability to inhibit the biological activity of PDGFBB using NR6R-3T3 mouse fibroblast cells. The ED50 for this effect is typically 3-8 mug/ml in the presence of 4 ng/ml rhPDGFBB.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for PDGFRB active protein

Background: Human platelet-derived growth factor receptor, beta polypeptide (PDGFRB), also called J03278, M21616, CD140B, JTK12, PDGF-R-beta and PDGFR, is receptor that binds specifically to PDGFB and has a tyrosine-protein kinase activity. Is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor (PDGF) family. The PDGFR/PDGF system includes two receptors (PDGFRA and PDGFRB) and four ligands (A, B, C and D). The receptors PDGFRA and PDGFRB are related in sequence and both are members of the class III subtype of receptor tyrosine kinases (RTKs). Other class III RTKs are CSF1R, KIT and FLT3. PDGF binding induces receptor homo- and heterodimerization and signal transduction. The expression of the alpha and beta receptors is independently regulated in various cell types. Recombinant soluble PDGFRB binds PDGF with high affinity and is potent PDGF antagonist. The ligands form either homo- or heterodimers (PDGF-AA, -AB, -BB, -CC, -DD). The four PDGFs are inactive in their monomeric forms. The PDGFs bind to the protein tyrosine kinase receptors PDGF receptor-alpha and -beta. These two receptor isoforms dimerize upon binding the PDGF dimer, leading to three possible receptor combinations, namely -alphaalpha, -betabeta and -alphabeta. PDGF-CC specifically interacts with PDGFR-alphaalpha and -alphabeta, but not with -betabeta, and thereby resembles PDGF-AB. PDGF-DD binds to PDGFR-betabeta with high affinity and to PDGFR-alphabeta to a much lower extent and is regarded as PDGFR-betabeta specific. PDGF-AA binds only to PDGFR-alphaalpha, while PDGF-BB is the only PDGF that can bind all three receptor combinations with high affinity.

Product Categories/Family for PDGFRB active protein

Cytokines; Growth Factors & Hormones; Human cell expressed recombinant proteins Proteins and Enzymes; Human cell expressed recombinant proteins Cytokines; Growth Factors & Hormones; InterleukinsProteins and Enzymes; Cytokines; Growth Factors & Hormones; C

NCBI and Uniprot Product Information

• NCBI GI #: 4505683
• NCBI GeneID: 5159
• NCBI Accession #: NP_002600.1
• NCBI GenBank Nucleotide #: NM_002609.3
• UniProt Accession #: P09619; Q8N5L4; B5A957
• Molecular Weight: This protein contains C-terminal polyhistidine tag and has a calculated MW of 57.1 kDa. As a result of glycosylation, DTT-reduced protein migrates as 85-95 kDa polypeptide in SDS-PAGE.
• NCBI Official Full Name: platelet-derived growth factor receptor beta
• NCBI Official Synonym Full Names: platelet-derived growth factor receptor, beta polypeptide
• NCBI Official Symbol: PDGFRB
• NCBI Official Synonym Symbols: IMF1; IBGC4; JTK12; PDGFR; CD140B; PDGFR1; PDGFR-1
• NCBI Protein Information: platelet-derived growth factor receptor beta
• UniProt Protein Name: Platelet-derived growth factor receptor beta
• Protein Family: Platelet-derived growth factor receptor
• UniProt Gene Name: PDGFRB
• UniProt Synonym Gene Names: PDGFR; PDGFR1; PDGF-R-beta; PDGFR-beta; PDGFR-1
• UniProt Entry Name: PGFRB_HUMAN

NCBI Description

This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophilia. [provided by RefSeq, Jul 2008]

Uniprot Description

PDGFRB: a receptor tyrosine kinase of the PDGFR family that binds members of the platelet-derived growth factor family. The identity of the growth factor bound determines whether the functional receptor is a homodimer or a heterodimer, composed of both PDGFR-alpha and -beta. Ligand binding induces receptor dimerization and autophosphorylation, thereby recruiting SH2-containing proteins including Grb2, Src, GAP, PTPN11, PI3 kinase, PLC-gamma and Nck. Regulates cell growth, actin reorganization, migration and differentiation. A variety of myeloproliferative disorders and cancers result from translocations that activate PDGFRbeta by fusion with proteins such as TEL/ETV6, H2, CEV14/TRP11, rabaptin 5, and huntington interacting protein 1. Gleevec treatment of TEL fusions has been successful. Overexpressed in metastatic medulloblastoma. Inhibitors: Gleevec, Sutent.

Protein type: Membrane protein, integral; Protein kinase, tyrosine (receptor); Protein kinase, TK; Oncoprotein; EC 2.7.10.1; Kinase, protein; TK group; PDGFR family

Chromosomal Location of Human Ortholog: 5q33.1

Cellular Component: lysosomal lumen; cell surface; focal adhesion; membrane; cytoplasmic membrane-bound vesicle; apical plasma membrane; cytoplasm; integral to membrane; plasma membrane; nucleus; intrinsic to plasma membrane

Molecular Function: protein binding; platelet activating factor receptor activity; platelet-derived growth factor beta-receptor activity; protein-tyrosine kinase activity; platelet-derived growth factor binding; platelet-derived growth factor receptor binding; phosphoinositide 3-kinase binding; platelet-derived growth factor receptor activity; protein kinase binding; ATP binding; receptor binding

Biological Process: positive regulation of phosphoprotein phosphatase activity; peptidyl-tyrosine phosphorylation; nerve growth factor receptor signaling pathway; wound healing; response to toxin; protein amino acid autophosphorylation; positive regulation of smooth muscle cell proliferation; positive regulation of collagen biosynthetic process; platelet-derived growth factor receptor signaling pathway; cardiac myofibril assembly; signal transduction; positive regulation of smooth muscle cell migration; response to estradiol stimulus; smooth muscle development; positive regulation of MAP kinase activity; positive regulation of cell proliferation; tissue homeostasis; phosphatidylinositol metabolic process; inner ear development; regulation of peptidyl-tyrosine phosphorylation; epidermal growth factor receptor signaling pathway; cell migration; response to retinoic acid; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; positive regulation of mitosis; positive regulation of chemotaxis; in utero embryonic development; adrenal gland development; positive regulation of phosphoinositide 3-kinase activity; positive regulation of phosphoinositide 3-kinase cascade; glycosaminoglycan biosynthetic process; G-protein coupled receptor protein signaling pathway; response to hyperoxia; response to hydrogen peroxide; skeletal morphogenesis; regulation of actin cytoskeleton organization and biogenesis; innate immune response; positive regulation of cell migration; negative regulation of apoptosis

Disease: Myeloproliferative Disorder, Chronic, With Eosinophilia; Myofibromatosis, Infantile, 1; Basal Ganglia Calcification, Idiopathic, 1; Basal Ganglia Calcification, Idiopathic, 4