Pro-Urokinase Active Protein | PLAU active protein

Pro-Urokinase, human recombinant

Cat. Num. AAA845347

• Gene Names: PLAU; ATF; QPD; UPA; URK; u-PA; BDPLT5
• Applications: SDS-Page
• Purity: >=90%
• Synonyms: Pro-Urokinase; human recombinant; Single chain Urokinase-type plasminogen activator (scuPA); Urokinase-type Plasminogen Activator uPA; PLAU.; PLAU active protein

• Host: E Coli
• Purity/Purification: >=90%
• Form/Format: Lyophilized from proprietary buffer. ; Appearance: Lyophilized powder
• Sequence Length: 414

• Applicable Applications for PLAU active protein: SDS-PAGE; HPLC
• Solubility/Reconstitution Instructions: Briefly spin down the vial and reconstitute in water to 0.5-1 mg/ml and store at -80 degree C.
• Activity (Specifications/test method): The enzyme is fully active as seen from its ability to cleave a fluorogenic substrate N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC).
• Biological Activity: >1200 mU/mg (1 U = Digestion of 1 umole of Z-GGR-AMC substrate in 1 min at 37 degree C.)
• Results: >1200 mU/mg (1 U = Digestion of 1 umole of Z-GGR-AMC substrate in 1 min at 37 degree C.)
• Unit Definition: 1 U = Digestion of 1 umole of Z-GGR-AMC substrate in 1 min at 37 degree C.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for PLAU active protein

Background: Urokinase or Urokinase-type plasminogen activator (uPA) is a serine protease (EC 3.4.21.73). It is secreted as a single-chain zymogen, pro-Urokinase, possessing little or no intrinsic enzymatic activity. The single chain zymogen is converted into the active two chain enzyme (tcuPA) by cleavage of the bond between Lys157 and Ile158. After activation, Urokinase specifically cleaves the proenzyme plasminogen to form the active enzyme plasmin. The active plasmin then catalyzes the breakdown of fibrin polymers of blood clots. Urokinase is involved in a number of biological functions including fibrinolysis, embryogenesis, cell migration, tissue remodeling, ovulation, and wound healing. Additionally, it is a potent marker of invasion and metastasis in a variety of human cancers associated with breast, stomach, colon, bladder, ovary, brain and endometrium.

Product Categories/Family for PLAU active protein

Proteins and Enzymes; Proteases; OtherProteins and Enzymes; Animal-Free Origin (AOF) Recombinant Proteins & EnzymesProteins and Enzymes; Proteins and Enzymes (A-Z)

NCBI and Uniprot Product Information

• NCBI GI #: 222537759
• NCBI GeneID: 5328
• NCBI Accession #: NP_001138503.1
• NCBI GenBank Nucleotide #: NM_001145031.1
• UniProt Accession #: P00749; Q15844; Q16618; Q53XS3; Q5SWW9; Q969W6; B4DPZ2
• Molecular Weight: 49.3 kDa
• NCBI Official Full Name: urokinase-type plasminogen activator isoform 2
• NCBI Official Synonym Full Names: plasminogen activator, urokinase
• NCBI Official Symbol: PLAU
• NCBI Official Synonym Symbols: ATF; QPD; UPA; URK; u-PA; BDPLT5
• NCBI Protein Information: urokinase-type plasminogen activator
• UniProt Protein Name: Urokinase-type plasminogen activator
• Protein Family: Urokinase-type plasminogen activator
• UniProt Gene Name: PLAU
• UniProt Synonym Gene Names: U-plasminogen activator; uPA
• UniProt Entry Name: UROK_HUMAN

NCBI Description

This gene encodes a serine protease involved in degradation of the extracellular matrix and possibly tumor cell migration and proliferation. A specific polymorphism in this gene may be associated with late-onset Alzheimer's disease and also with decreased affinity for fibrin-binding. This protein converts plasminogen to plasmin by specific cleavage of an Arg-Val bond in plasminogen. Plasmin in turn cleaves this protein at a Lys-Ile bond to form a two-chain derivative in which a single disulfide bond connects the amino-terminal A-chain to the catalytically active, carboxy-terminal B-chain. This two-chain derivative is also called HMW-uPA (high molecular weight uPA). HMW-uPA can be further processed into LMW-uPA (low molecular weight uPA) by cleavage of chain A into a short chain A (A1) and an amino-terminal fragment. LMW-uPA is proteolytically active but does not bind to the uPA receptor. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2009]

Uniprot Description

uPA: Specifically cleave the zymogen plasminogen to form the active enzyme plasmin. Defects in PLAU are the cause of Quebec platelet disorder (QPD). QPD is an autosomal dominant bleeding disorder due to a gain-of-function defect in fibrinolysis. Although affected individuals do not exhibit systemic fibrinolysis, they show delayed onset bleeding after challenge, such as surgery. The hallmark of the disorder is markedly increased PLAU levels within platelets, which causes intraplatelet plasmin generation and secondary degradation of alpha-granule proteins. Belongs to the peptidase S1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; EC 3.4.21.73; Protease; Secreted

Chromosomal Location of Human Ortholog: 10q22.2

Cellular Component: extracellular space; focal adhesion; cell surface; plasma membrane; extracellular region

Molecular Function: protein binding; serine-type endopeptidase activity

Biological Process: regulation of cell adhesion mediated by integrin; fibrinolysis; regulation of smooth muscle cell migration; smooth muscle cell migration; response to hypoxia; regulation of receptor activity; chemotaxis; signal transduction; proteolysis; blood coagulation; regulation of cell proliferation

Disease: Quebec Platelet Disorder; Alzheimer Disease