IL-15 active protein

Human CellExp IL-15, human recombinant

Cat. Num. AAA844993

• Gene Names: IL15; IL-15
• Applications: SDS-Page
• Purity: >=95%
• Synonyms: IL-15; Human CellExp IL-15; human recombinant; IL15; Interleukin-15; IL-15 active protein

• Host: HEK 293 cells
• Purity/Purification: >=95%
• Form/Format: Lyophilized from 0.22 mum filtered solution in 20 mM Tris, 100 mM NaCl, pH 8.0. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.; Appearance: Lyophilized powder
• Sequence Length: 162

• Applicable Applications for IL-15 active protein: SDS-PAGE
• Solubility/Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile deionized water to a concentration of 100 ug/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 month. For extended storage, it is recommended to store at -20 degree C.
• Endotoxin Level: < 0.1 ng/mug of protein (<1EU/mug) by LAL method
• Activity (Specifications/test method): The bio-activity was determined by dose-dependent stimulation of the proliferation of mouse CTLL-2 cells. The ED50 <0.5ng/ml, corresponding to a specific activity of >2X10^6 units/mg
• Biological Activity: The bio-activity was determined by dose-dependent stimulation of the proliferation of mouse CTLL-2 cells. The ED50 <0.5ng/ml, corresponding to a specific activity of >2X10^6 units/mg
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for IL-15 active protein

Background: Interleukin 15, also known as IL15, IL-15, and is a cytokine with structural similarity to IL-2. Like IL-2, IL-15 binds to and signals through the IL-2/IL-15 beta chain (CD122) and the common gamma chain (gamma-C, CD132). IL-15 is secreted by mononuclear phagocytes (and some other cells) following infection by virus (es). This cytokine induces cell proliferation of natural killer cells; cells of the innate immune system whose principal role is to kill virally infected cells. Interleukin 15 (IL-15) regulates T and natural killer (NK) cell activation and proliferation. Survival signals that maintain memory T cells in the absence of antigen are provided by IL-15. This cytokine is also implicated in NK cell development. In rodent lymphocytes, IL-15 prevents apoptosis by inducing an apoptosis inhibitor, BCL2L1/BCL-x (L). IL-15 has been shown to enhance the anti-tumor immunity of CD8+ T cells in pre-clinical models. A phase I clinical trial to evaluate the safety, dosing, and anti-tumor efficacy of IL-15 in patients with metastatic melanoma and renal cell carcinoma (kidney cancer) has begun to enroll patients at the National Institutes of Health

Product Categories/Family for IL-15 active protein

Cytokines; Growth Factors & Hormones; Human cell expressed recombinant proteins (NEW) Cytokines; Growth Factors & Hormones; InterleukinsCytokines; Growth Factors & Hormones; Cytokines & Growth Factors (A-Z) Proteins and Enzymes; Human cell expressed recom

NCBI and Uniprot Product Information

• NCBI GI #: 10835153
• NCBI GeneID: 3600
• NCBI Accession #: NP_000576.1
• NCBI GenBank Nucleotide #: NM_000585.4
• UniProt Accession #: P40933; O00440; O43512; Q495Z8; Q6FGX7; Q93058; Q9UBA3; D3DNZ2
• Molecular Weight: Calculated MW of 12.8 kDa with no tag. The predicted N-terminus is Asn49. DTT-reduced protein migrates as 16 kDa due to glycosylation.
• NCBI Official Full Name: interleukin-15 isoform 1 preproprotein
• NCBI Official Synonym Full Names: interleukin 15
• NCBI Official Symbol: IL15
• NCBI Official Synonym Symbols: IL-15
• NCBI Protein Information: interleukin-15
• UniProt Protein Name: Interleukin-15
• UniProt Gene Name: IL15
• UniProt Synonym Gene Names: IL-15
• UniProt Entry Name: IL15_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that regulates T and natural killer cell activation and proliferation. This cytokine and interleukine 2 share many biological activities. They are found to bind common hematopoietin receptor subunits, and may compete for the same receptor, and thus negatively regulate each other's activity. The number of CD8+ memory cells is shown to be controlled by a balance between this cytokine and IL2. This cytokine induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. Studies of the mouse counterpart suggested that this cytokine may increase the expression of apoptosis inhibitor BCL2L1/BCL-x(L), possibly through the transcription activation activity of STAT6, and thus prevent apoptosis. Alternatively spliced transcript variants of this gene have been reported. [provided by RefSeq, Feb 2011]

Uniprot Description

IL15: Cytokine that stimulates the proliferation of T- lymphocytes. Stimulation by IL-15 requires interaction of IL-15 with components of IL-2R, including IL-2R beta and probably IL-2R gamma but not IL-2R alpha. Belongs to the IL-15/IL-21 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Cell cycle regulation; Cytokine; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q31

Cellular Component: nucleoplasm; Golgi apparatus; extracellular space; cell surface; membrane; integral to plasma membrane; cytoplasm; extracellular region; endosome

Molecular Function: protein binding; hematopoietin/interferon-class (D200-domain) cytokine receptor binding; cytokine activity

Biological Process: positive regulation of interleukin-17 production; positive regulation of natural killer cell proliferation; positive regulation of immune response; NK T cell proliferation; negative regulation of smooth muscle cell proliferation; cell maturation; signal transduction; lymph node development; positive regulation of tyrosine phosphorylation of Stat3 protein; positive regulation of natural killer cell differentiation; extrathymic T cell selection; positive regulation of tissue remodeling; skeletal muscle atrophy; cell-cell signaling; natural killer cell differentiation; positive regulation of cell proliferation; regulation of T cell differentiation; immune response; positive regulation of T cell proliferation; regulation of defense response to virus by host; inflammatory response; hyaluronan metabolic process; positive regulation of inflammatory response; aging