PCSK9 active protein

Human CellExp PCSK9, human recombinant

Cat. Num. AAA844976

• Gene Names: PCSK9; FH3; PC9; NARC1; LDLCQ1; NARC-1; HCHOLA3
• Applications: SDS-Page
• Purity: >=97%
• Synonyms: PCSK9; Human CellExp PCSK9; human recombinant; FH3; HCHOLA3; LDLCQ1; NARC-1; NARC1; PC9; Proprotein convertase subtilisin/kexin type 9; PCSK9 active protein

• Host: HEK 293 cells
• Purity/Purification: >=97%
• Form/Format: Sterile filtered through a 0.22 micron filter. Lyophilized from 1 x PBS, pH 7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.; Appearance: Lyophilized powder
• Sequence Length: 692

• Applicable Applications for PCSK9 active protein: SDS-PAGE
• Solubility/Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 100 ug/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 month. For extended storage, it is recommended to store at -20 degree C.
• Endotoxin Level: < 0.1 ng/mug of protein (<1EU/mug) by LAL method
• Activity (Specifications/test method): Measured by its ability to bind with human LDLR in a functional ELISA Immobilized recombinant human PCSK9 at 10 mug/ml (100 mul/well) can bind biotinylated human LDLR. The ED50 for this effect is typically 15 ng/ml.
• Biological Activity: Measured by its ability to bind with human LDLR in a functional ELISA Immobilized recombinant human PCSK9 at 10 mug/ml (100 mul/well) can bind biotinylated human LDLR. The ED50 for this effect is typically 15 ng/ml.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for PCSK9 active protein

Background: Proprotein convertase subtilisin/kexin type 9 (PCSK9), is an enzyme which in humans is encoded by the PCSK9 gene. This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).

Product Categories/Family for PCSK9 active protein

Cytokines; Growth Factors & Hormones; Human cell expressed recombinant proteins (NEW) Cytokines; Growth Factors & Hormones; Cytokines & Growth Factors (A-Z) Proteins and Enzymes; Human cell expressed recombinant proteins (NEW) Proteins and Enzymes; Cytoki

NCBI and Uniprot Product Information

• NCBI GI #: 31317307
• NCBI GeneID: 255738
• NCBI Accession #: NP_777596.2
• NCBI GenBank Nucleotide #: NM_174936.3
• UniProt Accession #: Q8NBP7; Q5PSM5; Q5SZQ2; A8T640; C0JYY9
• Molecular Weight: This protein fused with polyhistidine tag at the C-terminus, has a calculated MW of 75.1 kDa. The predicted N-terminus is Gln 31. DTT-reduced Protein migrates as 20 kDa and 62 kDa due to glycosylation and proteolytic digestion.
• NCBI Official Full Name: proprotein convertase subtilisin/kexin type 9 preproprotein
• NCBI Official Synonym Full Names: proprotein convertase subtilisin/kexin type 9
• NCBI Official Symbol: PCSK9
• NCBI Official Synonym Symbols: FH3; PC9; NARC1; LDLCQ1; NARC-1; HCHOLA3
• NCBI Protein Information: proprotein convertase subtilisin/kexin type 9
• UniProt Protein Name: Proprotein convertase subtilisin/kexin type 9
• Protein Family: Proprotein convertase subtilisin/kexin
• UniProt Gene Name: PCSK9
• UniProt Synonym Gene Names: NARC1; NARC-1; PC9
• UniProt Entry Name: PCSK9_HUMAN

NCBI Description

This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The encoded protein undergoes an autocatalytic processing event with its prosegment in the ER and is constitutively secreted as an inactive protease into the extracellular matrix and trans-Golgi network. It is expressed in liver, intestine and kidney tissues and escorts specific receptors for lysosomal degradation. It plays a role in cholesterol and fatty acid metabolism. Mutations in this gene have been associated with autosomal dominant familial hypercholesterolemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]

Uniprot Description

PCSK9: Crucial player in the regulation of plasma cholesterol homeostasis. Binds to low-density lipid receptor family members: low density lipoprotein receptor (LDLR), very low density lipoprotein receptor (VLDLR), apolipoprotein E receptor (LRP1/APOER) and apolipoprotein receptor 2 (LRP8/APOER2), and promotes their degradation in intracellular acidic compartments. Acts via a non-proteolytic mechanism to enhance the degradation of the hepatic LDLR through a clathrin LDLRAP1/ARH-mediated pathway. May prevent the recycling of LDLR from endosomes to the cell surface or direct it to lysosomes for degradation. Can induce ubiquitination of LDLR leading to its subsequent degradation. Inhibits intracellular degradation of APOB via the autophagosome/lysosome pathway in a LDLR-independent manner. Involved in the disposal of non-acetylated intermediates of BACE1 in the early secretory pathway. Inhibits epithelial Na(+) channel (ENaC)-mediated Na(+) absorption by reducing ENaC surface expression primarily by increasing its proteasomal degradation. Regulates neuronal apoptosis via modulation of LRP8/APOER2 levels and related anti-apoptotic signaling pathways. Defects in PCSK9 are the cause of hypercholesterolemia autosomal dominant type 3 (HCHOLA3). A familial condition characterized by elevated circulating cholesterol contained in either low-density lipoproteins alone or also in very-low-density lipoproteins. Belongs to the peptidase S8 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; Secreted; Cell development/differentiation; Secreted, signal peptide; EC 3.4.21.-

Chromosomal Location of Human Ortholog: 1p32.3

Cellular Component: Golgi apparatus; extracellular space; extrinsic to external side of plasma membrane; cell surface; rough endoplasmic reticulum; perinuclear region of cytoplasm; lysosome; endoplasmic reticulum; early endosome; late endosome; cytoplasm; plasma membrane; ER to Golgi transport vesicle

Molecular Function: very-low-density lipoprotein binding; sodium channel inhibitor activity; protein binding; protein self-association; low-density lipoprotein receptor binding; serine-type endopeptidase activity; low-density lipoprotein binding; apolipoprotein binding; apolipoprotein receptor binding

Biological Process: cholesterol metabolic process; lysosomal transport; apoptosis; positive regulation of receptor internalization; lipoprotein metabolic process; regulation of low-density lipoprotein receptor catabolic process; cellular response to starvation; proteolysis; liver development; neuron differentiation; protein autoprocessing; cholesterol homeostasis; triacylglycerol metabolic process; cellular response to insulin stimulus; neurogenesis; phospholipid metabolic process; positive regulation of neuron apoptosis; regulation of neuron apoptosis; negative regulation of receptor recycling; low-density lipoprotein receptor catabolic process; regulation of receptor activity; kidney development

Disease: Hypercholesterolemia, Autosomal Dominant, 3