TFPI2 active protein

Human CellExp TFPI2, human recombinant

Cat. Num. AAA844529

• Gene Names: TFPI2; PP5; REF1; TFPI-2
• Applications: SDS-Page
• Purity: >=95%
• Synonyms: TFPI2; Human CellExp TFPI2; human recombinant; PP5; REF1; TFPI2 active protein

• Host: HEK293 cells
• Purity/Purification: >=95%
• Form/Format: Lyophilized from 0.22 mum filtered solution in 20 mM MES and 100 mM NaCl, pH 6.5. Normally Mannitol or Trehalose is added as protectants before lyophilization.; Appearance: Lyophilized
• Sequence Length: 224

• Applicable Applications for TFPI2 active protein: SDS-PAGE
• Solubility/Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 ug/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 month. For extended storage, it is recommended to store at -20 degree C.
• Endotoxin Level: <1 EU/mug by LAL method
• Biological Activity: Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca - RPKPVE - Nval - WRK (Dnp) - NH2. The IC50 value is < 2.5 nM, as measured in 100muL reaction mixture containing 1.25 ng trypsin, 10 muM substrate, 50 mM Tris, 10 mM CaCl2, 0.15 M NaCl, 0.05% Brij-35, pH 7.5.
• Results: Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca - RPKPVE - Nval - WRK (Dnp) - NH2. The IC50 value is < 2.5 nM, as measured in 100muL reaction mixture containing 1.25 ng trypsin, 10 muM substrate, 50 mM Tris, 10 mM CaCl2, 0.15 M NaCl, 0.05% Brij-35, pH 7.5.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for TFPI2 active protein

Background: Tissue Factor Pathway Inhibitor 2 (TFPI-2), also known as placental protein 5 (PP5) and retinal pigment epithelial cell factor 1 (REF-1), is a member of the Kunitz-type serine proteinase inhibitor family, is a structural homologue of tissue factor pathway inhibitor (TFPI). TFPI2 is highly abundant in the full-term placenta and widely expressed in umbilical vein endothelial cells, liver, placenta, heart, pancreas, and maternal serum at advanced pregnancy. TFPI-2 may play a role in the regulation of plasmin-mediated matrix remodeling. Inhibits trypsin, plasmin, factor VIIa/tissue factor and weakly factor Xa and has no effect on thrombin. Reduced synthesis of TFPI-2 has been related to numerous pathophysiological processes such as inflammation, angiogenesis, atherosclerosis, retinal degeneration and tumor growth / metastasis.

Product Categories/Family for TFPI2 active protein

Proteins and Enzymes; Human cell expressed recombinant proteinsProteins and Enzymes; Proteins and Enzymes (A-Z) Proteins and Enzymes; Other Proteins & Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 401871059
• NCBI GeneID: 7980
• NCBI Accession #: NP_001257932.1
• NCBI GenBank Nucleotide #: NM_001271003.1
• UniProt Accession #: P48307; Q66ME8; Q8NAK6; Q9UC86
• Molecular Weight: This protein is fused with 6×His tag at the N-terminus, has a calculated MW of 22.7 kDa. The predicted N-terminus is Asp 23. DTT-reduced Protein migrates as 26-32 kDa due to glycosylation.
• NCBI Official Full Name: tissue factor pathway inhibitor 2 isoform 2
• NCBI Official Synonym Full Names: tissue factor pathway inhibitor 2
• NCBI Official Symbol: TFPI2
• NCBI Official Synonym Symbols: PP5; REF1; TFPI-2
• NCBI Protein Information: tissue factor pathway inhibitor 2
• UniProt Protein Name: Tissue factor pathway inhibitor 2
• Protein Family: Tissue factor pathway inhibitor
• UniProt Gene Name: TFPI2
• UniProt Synonym Gene Names: TFPI-2; PP5
• UniProt Entry Name: TFPI2_HUMAN

NCBI Description

This gene encodes a member of the Kunitz-type serine proteinase inhibitor family. The protein can inhibit a variety of serine proteases including factor VIIa/tissue factor, factor Xa, plasmin, trypsin, chymotryspin and plasma kallikrein. This gene has been identified as a tumor suppressor gene in several types of cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

Uniprot Description

TFPI2: May play a role in the regulation of plasmin-mediated matrix remodeling. Inhibits trypsin, plasmin, factor VIIa/tissue factor and weakly factor Xa. Has no effect on thrombin.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 7q22

Cellular Component: proteinaceous extracellular matrix; nucleus

Molecular Function: serine-type endopeptidase inhibitor activity; extracellular matrix structural constituent

Biological Process: blood coagulation