Apo-SAA Active Protein | SAA1 active protein

Apo-SAA, human recombinant

Cat. Num. AAA844510

• Gene Names: SAA1; SAA; PIG4; SAA2; TP53I4
• Applications: SDS-Page
• Purity: >98% by SDS-PAGE and HPLC analyses. Endotoxin level is <0.1 ng/ug of Apo-SAA.
• Synonyms: Apo-SAA; human recombinant; Serum amyloid A protein; SAA; Amyloid protein A; Amyloid fibril protein AA; Apolipoprotein; apolipoproteins; SAA1; SAA2; PIG4; TP53I4; MGC111216; ; MGC111216;; SAA1 active protein

• Host: E Coli
• Purity/Purification: >98% by SDS-PAGE and HPLC analyses. Endotoxin level is <0.1 ng/ug of Apo-SAA.
• Form/Format: Lyophilized with no additives ; Appearance: Lyophilized protein
• Sequence Length: 122

• Applicable Applications for SAA1 active protein: SDS-PAGE; HPLC
• Reconstitution: Centrifuge the vial prior to opening. Reconstitute in 0.1% acetic acid to a concentration of 1 ug/ul. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.
• Biological Activity: For most in vitro applications, Apo-SAA exerts its biological activity in the concentration range of 10-50 ug/ml.
• Preparation and Storage: The lyophilized human Apo-SAA is best-stored desiccated below 0°C. Reconstituted Apo-SAA should be stored in working aliquots at -20 degree C.

SDS-PAGE

Related Product Information for SAA1 active protein

Background: Human apo-SAA is a 104 amino acid polypeptide that circulates primarily in association with high-density lipoproteins (HDL). The level of apo-SAA, normally 1-5 ug/ml in plasma, increases 500-1000 fold within 24 hours of an inflammatory stimulus and, under these conditions, is the most abundant HDL apo-lipoprotein. The human SAA gene codes for a 122 amino acid polypeptide, which contains an 18 amino acid N-terminal signal sequence.

NCBI and Uniprot Product Information

• NCBI GI #: 40316912
• NCBI GeneID: 6288
• NCBI Accession #: NP_000322.2
• NCBI GenBank Nucleotide #: NM_000331.4
• UniProt Accession #: P0DJI8; P02735; P02736; P02737; Q16730; Q16834; Q16835; Q16879; Q3KRB3; Q6FG67; Q96QN0; Q9UCK9
• Molecular Weight: ~11.5 kDa
• NCBI Official Full Name: serum amyloid A-1 protein preproprotein
• NCBI Official Synonym Full Names: serum amyloid A1
• NCBI Official Symbol: SAA1
• NCBI Official Synonym Symbols: SAA; PIG4; SAA2; TP53I4
• NCBI Protein Information: serum amyloid A-1 protein
• UniProt Protein Name: Serum amyloid A-1 protein
• Protein Family: Amyloid protein
• UniProt Gene Name: SAA1
• UniProt Synonym Gene Names: SAA
• UniProt Entry Name: SAA1_HUMAN

NCBI Description

This gene encodes a member of the serum amyloid A family of apolipoproteins. The encoded protein is a major acute phase protein that is highly expressed in response to inflammation and tissue injury. This protein also plays an important role in HDL metabolism and cholesterol homeostasis. High levels of this protein are associated with chronic inflammatory diseases including atherosclerosis, rheumatoid arthritis, Alzheimer's disease and Crohn's disease. This protein may also be a potential biomarker for certain tumors. Alternate splicing results in multiple transcript variants that encode the same protein. A pseudogene of this gene is found on chromosome 11.[provided by RefSeq, Jun 2012]

Uniprot Description

SAA1: Major acute phase reactant. Apolipoprotein of the HDL complex. Reactive, secondary amyloidosis is characterized by the extracellular accumulation in various tissues of the SAA1 protein. These deposits are highly insoluble and resistant to proteolysis; they disrupt tissue structure and compromise function. Elevated serum SAA1 protein levels may be associated with lung cancer. Belongs to the SAA family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11p15.1

Cellular Component: extracellular region

Molecular Function: heparin binding; G-protein-coupled receptor binding

Biological Process: positive regulation of interleukin-1 secretion; neutrophil chemotaxis; receptor-mediated endocytosis; platelet activation; elevation of cytosolic calcium ion concentration; regulation of protein secretion; positive regulation of cell adhesion; negative regulation of inflammatory response; acute-phase response; innate immune response; lymphocyte chemotaxis; macrophage chemotaxis; positive regulation of cytokine secretion