GDA active protein

GDA, human recombinant

Cat. Num. AAA844204

• Gene Names: GDA; CYPIN; GUANASE; NEDASIN
• Applications: SDS-Page
• Purity: >=90%
• Synonyms: GDA; human recombinant; Guanine deaminase; CYPIN; GUANASE; NEDASIN; GDA active protein

• Host: E Coli
• Purity/Purification: >=90%
• Form/Format: 1 mg/ml solution 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol and 1 mM DTT.; Appearance: Liquid
• Concentration: 1 mg/ml (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMCAAQMP PLAHIFRGTF VHSTWTCPME VLRDHLLGVS DSGKIVFLEE ASQQEKLAKE WCFKPCEIRE LSHHEFFMPG LVDTHIHASQ YSFAGSSIDL PLLEWLTKYT FPAEHRFQNI DFAEEVYTRV VRRTLKNGTT TACYFATIHT DSSLLLADIT DKFGQRAFVG KVCMDLNDTF PEYKETTEES IKETERFVSE MLQKNYSRVK PIVTPRFSLS CSETLMGELG NIAKTRDLHI QSHISENRDE VEAVKNLYPS YKNYTSVYDK NNLLTNKTVM AHGCYLSAEE LNVFHERGAS IAHCPNSNLS LSSGFLNVLE VLKHEVKIGL GTDVAGGYSY SMLDAIRRAV MVSNILLINK VNEKSLTLKE VFRLATLGGS QALGLDGEIG NFEVGKEFDA ILINPKASDS PIDLFYGDFF GDISEAVIQK FLYLGDDRNI EEVYVGGKQV VPFSSSV
• Sequence Length: 471

• Applicable Applications for GDA active protein: SDS-PAGE
• Biological Activity: Specific activity is > 0.9 unit/ml, in which one unit will deaminate 1.0 umole of guanine to xanthine per minute at pH 8.0 at 25 degree C.
• Results: Specific activity is > 0.9 unit/ml
• Unit Definition: One unit will deaminate 1.0 umole of guanine to xanthine per minute at pH 8.0 at 25 degree C.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for GDA active protein

Background: GDA is an enzyme responsible for the hydrolytic deamination of guanine. Studies in rat ortholog suggest this gene plays a role in microtubule assembly. Multiple transcript variants encoding different isoforms have been found for this gene. Recombinant human GDA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Product Categories/Family for GDA active protein

Proteins and Enzymes; Proteins and Enzymes (A-Z)

NCBI and Uniprot Product Information

• NCBI GI #: 335335014
• NCBI GeneID: 9615
• NCBI Accession #: NP_001229434.1
• NCBI GenBank Nucleotide #: NM_001242505.2
• UniProt Accession #: Q9Y2T3; Q5SZC7; Q9H335; Q9ULG2; B4DTY5
• Molecular Weight: 53 kDa (477 aa, 1-454 aa + His Tag)
• NCBI Official Full Name: guanine deaminase isoform a
• NCBI Official Synonym Full Names: guanine deaminase
• NCBI Official Symbol: GDA
• NCBI Official Synonym Symbols: CYPIN; GUANASE; NEDASIN
• NCBI Protein Information: guanine deaminase
• UniProt Protein Name: Guanine deaminase
• UniProt Gene Name: GDA
• UniProt Synonym Gene Names: KIAA1258; Guanase; Guanine aminase; GAH
• UniProt Entry Name: GUAD_HUMAN

NCBI Description

This gene encodes an enzyme responsible for the hydrolytic deamination of guanine. Studies in rat ortholog suggest this gene plays a role in microtubule assembly. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

Uniprot Description

GDA: Catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia. Belongs to the ATZ/TRZ family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.5.4.3; Hydrolase; Nucleotide Metabolism - purine

Chromosomal Location of Human Ortholog: 9q21.13

Cellular Component: intracellular; cytosol

Molecular Function: guanine deaminase activity; zinc ion binding

Biological Process: nervous system development; nucleobase, nucleoside and nucleotide metabolic process; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; purine nucleotide catabolic process; guanine catabolic process; purine base metabolic process