Kallikrein Protein | KLKB1 protein

Kallikrein, Human Plasma

Cat. Num. AAA844072

• Gene Names: KLKB1; PPK; KLK3; PKKD
• Applications: SDS-Page
• Purity: >=95%
• Synonyms: Kallikrein; Human Plasma; Fletcher factor; Kininogenin; Plasma prekallikrein; KLKB1 protein

• Host: Human Plasma
• Purity/Purification: >=95%
• Form/Format: Frozen in 20 mM Tris-HCl, pH 7.8, with 100 mM NaCl; Appearance: Liquid
• Sequence Length: 638

• Applicable Applications for KLKB1 protein: SDS-PAGE; HPLC
• Activity (Specifications/test method): 15 units/mg
• Unit Definition: One unit is defined as the amount of enzyme that hydrolyzes one umol of D-Pro-Phe-Arg-pNA per minute at 25 degree C, pH 7.8.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -70 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for KLKB1 protein

Background: A serine protease that releases bradykinin from kininogen. Present in plasma as an inactive precursor prokallikrein.

Product Categories/Family for KLKB1 protein

Proteins and Enzymes; Native ProteinsProteins & Enzymes; Other Proteins & Enzymes and Proteins & Enzymes (A-Z)

NCBI and Uniprot Product Information

• NCBI GI #: 78191798
• NCBI GeneID: 3818
• NCBI Accession #: NP_000883.2
• NCBI GenBank Nucleotide #: NM_000892.3
• UniProt Accession #: P03952; Q17RE8; Q17RE9; Q4W5C3; A6NH96; B2R8H9
• Molecular Weight: 86.0 - 88.0 kDa
• NCBI Official Full Name: plasma kallikrein preproprotein
• NCBI Official Synonym Full Names: kallikrein B, plasma (Fletcher factor) 1
• NCBI Official Symbol: KLKB1
• NCBI Official Synonym Symbols: PPK; KLK3; PKKD
• NCBI Protein Information: plasma kallikrein
• UniProt Protein Name: Plasma kallikrein
• Protein Family: Kallikrein
• UniProt Gene Name: KLKB1
• UniProt Synonym Gene Names: KLK3
• UniProt Entry Name: KLKB1_HUMAN

NCBI Description

Plasma prekallikrein is a glycoprotein that participates in the surface-dependent activation of blood coagulation, fibrinolysis, kinin generation and inflammation. It is synthesized in the liver and secreted into the blood as a single polypeptide chain. Plasma prekallikrein is converted to plasma kallikrein by factor XIIa by the cleavage of an internal Arg-Ile bond. Plasma kallikrein therefore is composed of a heavy chain and a light chain held together by a disulphide bond. The heavy chain originates from the amino-terminal end of the zymogen and contains 4 tandem repeats of 90 or 91 amino acids. Each repeat harbors a novel structure called the apple domain. The heavy chain is required for the surface-dependent pro-coagulant activity of plasma kallikrein. The light chain contains the active site or catalytic domain of the enzyme and is homologous to the trypsin family of serine proteases. Plasma prekallikrein deficiency causes a prolonged activated partial thromboplastin time in patients. [provided by RefSeq, Jul 2008]

Uniprot Description

KLKB1: The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin. Defects in KLKB1 are the cause of prekallikrein deficiency (PKK deficiency); also known as Fletcher factor deficiency. This disorder is a blood coagulation defect. Belongs to the peptidase S1 family. Plasma kallikrein subfamily.

Protein type: Secreted; Secreted, signal peptide; Protease; EC 3.4.21.34

Chromosomal Location of Human Ortholog: 4q35

Cellular Component: extracellular space; extracellular region; plasma membrane

Molecular Function: protein binding; serine-type endopeptidase activity

Biological Process: extracellular matrix disassembly; fibrinolysis; extracellular matrix organization and biogenesis; positive regulation of fibrinolysis; zymogen activation; proteolysis; blood coagulation; blood coagulation, intrinsic pathway; Factor XII activation; plasminogen activation

Disease: Prekallikrein Deficiency