Testing Data
Mouse Osteopontin Monoclonal Antibody | anti-SPP1 antibody
Anti-Osteopontin (NT) (Clone 2F10)
Appearance: Colorless liquid
Shelf Life: 12 months
Testing Data
Testing Data
Background: Human Osteopontin (OPN) is a negatively charged hydrophilic protein of 314 amino acids and is subject to significant post translational modifications (PTM) including phosphorylation and glycosylation. Due to its acidic nature and PTMs, OPN runs anomalously by SDS-PAGE. Althomugh its mass is 35kD, apparent molecular weights may range up to 75kD. In addition, OPN is subject to proteolytic modification into smaller molecular weight fragments. The matricellular protein osteopontin binds to cell surface receptors and is secreted into many body fluids including milk, blood and urine, depending on the organ of origin. This makes osteopontin an ideal candidate for being a biomarker as the secreted form is easily obtained in throwaway fluids, and mimics the cellular environment from which it is released. Osteopontin is important in immune responses and inflammation as well as bone generation and remodeling. In autistic children, serum levels of osteopontin are correlated to the severity of disease, probably due to a brain inflammation pattern in these children. In aortic valve sclerosis and stenosis, increased levels of secreted osteopontin are also noted. Osteopontin has also been smuggested as a cancer biomarker, since it is associated with tumor formation, progression and metastasis. In bone and tooth formation osteopontin is known to be a negative regulator of parathyroid hormone-related protein receptor, which induces osteogenesis. Without appropriate levels of osteopontin, bone growth continues unregulated, and leads to specific bone cancers. In short, osteopontin is a strong marker for bone growth, inflammation and certain cancers. The newly exposed SVVYG epitope on the N-terminal fragment has also been shown to participate in cell adhesion.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
Uniprot Description
osteopontin: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Belongs to the osteopontin family. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 4q22.1
Cellular Component: extracellular space; cell projection; perinuclear region of cytoplasm; apical part of cell; extracellular region
Molecular Function: protein binding; extracellular matrix binding; cytokine activity
Biological Process: osteoblast differentiation; extracellular matrix disassembly; neutrophil chemotaxis; extracellular matrix organization and biogenesis; response to vitamin D; response to steroid hormone stimulus; biomineral formation; negative regulation of collateral sprouting of intact axon in response to injury; positive regulation of bone resorption; decidualization; inflammatory response; cell adhesion; embryo implantation
Research Articles on SPP1
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Product Notes
The SPP1 spp1 (Catalog #AAA840050) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Osteopontin (NT) (Clone 2F10) reacts with Recognizes native Human Osteopontin from purified breast milk. Specific for the N-terminal fragment of full-length Osteopontin. and may cross-react with other species as described in the data sheet. AAA Biotech's Osteopontin can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Researchers should empirically determine the suitability of the SPP1 spp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Osteopontin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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