Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human brain tissue using DARP32 antibody)
Rabbit DARP32 Polyclonal Antibody | anti-DARP32 antibody
DARP32 antibody
Applications
Western Blot, Immunohistochemistry, ELISA
Synonyms
DARP32; Polyclonal Antibody; DARP32 antibody; Polyclonal DARP32; Anti-DARP32; Dopamine- and cAMregulated neuronal phosphoprotein; IPPD; Neuronal phosphoprotein DARP32; PPP1R1B; anti-DARP32 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Specificity
DARP32 antibody detects endogenous levels of total DARP32 protein
Form/Format
Purified IgG in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1 mg/ml (varies by lot)
Applicable Applications for anti-DARP32 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1000
IHC: 1:50-100
ELISA: 1:40000
IHC: 1:50-100
ELISA: 1:40000
Biological Significance
DARP32 was identified initially as a major target for dopamine and protein kinase A (PKA) in striatum. However, recent advances now indicate that regulation of the state of DARPP-32 phosphorylation provides a mechanism for integrating information arriving at dopaminoceptive neurons, in multiple brain regions, via a variety of neurotransmitters, neuromodulators, neuropeptides, and steroid hormones.
Cross-Reactivity
Mouse, Rat
Immunogen
DARP32 antibody was raised in Rabbit using a synthesized non-phospho derived from human DARP32 around the phosphorylation site of threonine 75 (A-Y-TP) as the immunogen
Preparation and Storage
Store at -20 degree C in small aliquots. Avoid repeated freeze-thaw cycles
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human brain tissue using DARP32 antibody)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human brain tissue using DARP32 antibody)
Related Product Information for anti-DARP32 antibody
Rabbit polyclonal DARP32 antibody
Product Categories/Family for anti-DARP32 antibody
Similar Products
Product Notes
The DARP32 (Catalog #AAA839311) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's DARP32 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1000 IHC: 1:50-100 ELISA: 1:40000. Researchers should empirically determine the suitability of the DARP32 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DARP32, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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