Western Blot (WB)
(Western Blot analysis of Daudi cell lysates prepared under reducing conditions using Pax5 antibody at 2 ug/ml and visualized using Rat IgG HRP antibody)
Rat Pax5 Monoclonal Antibody | anti-Pax5 antibody
Pax5 antibody
Applications
Functional Assay
Purity
Pax5 antibody was purified by affinity chromatography.
Synonyms
Pax5; Monoclonal Antibody; Pax5 antibody; Monoclonal Pax5; Anti-Pax5; Pax-5; Pax 5; anti-Pax5 antibody
Host
Rat
Clonality
Monoclonal
Isotype
IgG2a kappa
Clone Number
1H9
Specificity
Mouse, Human
Purity/Purification
Pax5 antibody was purified by affinity chromatography.
Form/Format
Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Applicable Applications for anti-Pax5 antibody
Functional Assay
Application Notes
This reagent has been tested in bioassays using the MCF-7 cell line. The ED50 measured in the dose-dependent induction of MCF-7 cell proliferation assay is typically 0.85 ng/ml, corresponding to a specific activity of approximately 1 x10 Units/mg.
Biological Significance
The monoclonal antibody 1H9 recognizes both mouse and human Pax5. Pax5, also known as BSAP, is a member of the paired box family of transcription factors. Pax5 is the only member of the pax family of transcription factors that is expressed in hematopoietic cells. During embryogenesis, Pax5 is transiently expressed in the brain of mice and in the mesencephalon and spinal cord of humans. Its expression is upregulated early in B cell development at the time of B cell commitment and is maintained throughout most subsequent stages. Suppression of Pax5 is essential for expression of Blimp-1 and the terminal differentiation of plasma cells. In the spleen, expression of Pax5 is higher in marginal zone B cells than in other B cells, especially the transition 1 stage. In addition to its role in B cell development, Pax5 also affects VH-DJH heavy chain recombination as well as influencing the expression of many other B and non-B cell related proteins. Pax5 expression is correlated with many neoplasms. In diffuse large B cell lymphomas and non-Hodgkin lymphomas Pax5 is often mutated while in B-cell ALL, expression levels are high. Additionally, translocation with elastin, IGH, ETV6, FOXP1, and EVI3 have been identified. science recommends that the Foxp3 Buffers be used for optimal results when using this antibody for intracellular staining and flow cytometric analysis.
Preparation and Storage
Store at 2-8 degree C. Do not freeze. Protect from light.
Western Blot (WB)
(Western Blot analysis of Daudi cell lysates prepared under reducing conditions using Pax5 antibody at 2 ug/ml and visualized using Rat IgG HRP antibody)
Western Blot (WB)
(Western Blot analysis of Daudi cell lysates prepared under reducing conditions using Pax5 antibody at 2 ug/ml and visualized using Rat IgG HRP antibody)
Related Product Information for anti-Pax5 antibody
Rat monoclonal Pax5 antibody
Product Categories/Family for anti-Pax5 antibody
Similar Products
Product Notes
The Pax5 (Catalog #AAA838727) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Pax5 can be used in a range of immunoassay formats including, but not limited to, Functional Assay. This reagent has been tested in bioassays using the MCF-7 cell line. The ED50 measured in the dose-dependent induction of MCF-7 cell proliferation assay is typically 0.85 ng/ml, corresponding to a specific activity of approximately 1 x10 Units/mg. Researchers should empirically determine the suitability of the Pax5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Pax5, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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