Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of HEK293T cells transfected with either recombinant PON1 protein (red) or empty vector (blue) stained using PON1 antibody)
Mouse PON1 Monoclonal Antibody | anti-PON1 antibody
PON1 antibody
Applications
Flow Cytometry, Functional Assay, Western Blot
Purity
PON1 antibody was purified by affinity chromatography.
Synonyms
PON1; Monoclonal Antibody; PON1 antibody; Monoclonal PON1; Anti-PON1; Paraoxonase 1; ESA; PON; PON 1; PON-1; anti-PON1 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2A9
Purity/Purification
PON1 antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
500 ug-1 mg/ml (varies by lot)
Applicable Applications for anti-PON1 antibody
Flow Cytometry (FC/FACS), Western Blot (WB)
Application Notes
FC: 1:100
WB: 1:500-2000
WB: 1:500-2000
Biological Significance
PON1 hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. It is capable of hydrolyzing a broad spectrum of organophosphate substrates and a number of aromatic carboxylic acid esters. It may mediate an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation.
Cross-Reactivity
Human, Monkey
Immunogen
PON1 antibody was raised in mouse using a full length recombinant protein of human PON1 (NP_000437) produced in HEK293T cells, as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of HEK293T cells transfected with either recombinant PON1 protein (red) or empty vector (blue) stained using PON1 antibody)
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of HEK293T cells transfected with either recombinant PON1 protein (red) or empty vector (blue) stained using PON1 antibody)
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant PON1 protein (Right) or empty vector (Left) detected with PON1 antibody)
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant PON1 protein (Right) or empty vector (Left) detected with PON1 antibody)
Related Product Information for anti-PON1 antibody
Mouse monoclonal PON1 antibody
Product Categories/Family for anti-PON1 antibody
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Product Notes
The PON1 (Catalog #AAA838395) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's PON1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Western Blot (WB). FC: 1:100 WB: 1:500-2000. Researchers should empirically determine the suitability of the PON1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PON1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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