Mouse PRKAR2A Monoclonal Antibody | anti-PRKAR2A antibody

PRKAR2A antibody

Cat. Num. AAA838334

• Gene Names: PRKAR2A; PKR2; PRKAR2
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: PRKAR2A antibody was purified by affinity chromatography.
• Synonyms: PRKAR2A; Monoclonal Antibody; PRKAR2A antibody; Monoclonal PRKAR2A; Anti-PRKAR2A; Protein kinase cAMP-dependent regulatory type II alpha; PKR2; PRKAR2; MGC3606; cAMP-dependent protein kinase type II-alpha regulatory subunit; PRKA 2Ra; PRKA-2Ra; anti-PRKAR2A antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 1A7
• Purity/Purification: PRKAR2A antibody was purified by affinity chromatography.
• Form/Format: Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 500 ug-1 mg/ml (varies by lot)
• Sequence Length: 382

• Applicable Applications for anti-PRKAR2A antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC: 1:100; IF: 1:100; IHC: 1:50; WB: 1:2000
• Biological Significance: cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating thecAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The in active kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the in active holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellularlocalization of cAMP-dependent protein kinase.
• Cross-Reactivity: Human
• Immunogen: PRKAR2A antibody was raised in mouse using a full length recombinant protein of human PRKAR2A (NP_004148) produced in HEK293T cells, as the immunogen.
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Immunofluorescence (IF)

(Immunofluorescent staining of COS7 cells transiently transfected with recombinant PRKAR2A protein using PRKAR2A antibody)

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant PRKAR2A protein (Right) or empty vector (Left) detected with PRKAR2A antibody)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of PRKAR2A protein in paraffin embedded Human kidney tissue using PRKAR2A antibody)

Related Product Information for anti-PRKAR2A antibody

Mouse monoclonal PRKAR2A antibody

Product Categories/Family for anti-PRKAR2A antibody

Proteases; Inhibitors; & Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 12803843
• NCBI GeneID: 5576
• Molecular Weight: 43,067 Da
• NCBI Official Full Name: PRKAR2A protein
• NCBI Official Synonym Full Names: protein kinase, cAMP-dependent, regulatory, type II, alpha
• NCBI Official Symbol: PRKAR2A
• NCBI Official Synonym Symbols: PKR2; PRKAR2
• NCBI Protein Information: cAMP-dependent protein kinase type II-alpha regulatory subunit
• UniProt Protein Name: cAMP-dependent protein kinase type II-alpha regulatory subunit
• Protein Family: cAMP-dependent protein kinase
• UniProt Gene Name: PRKAR2A
• UniProt Synonym Gene Names: PKR2; PRKAR2
• UniProt Entry Name: KAP2_HUMAN

NCBI Description

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of cAMP-dependent protein kinase. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER). [provided by RefSeq, Jul 2008]

Uniprot Description

PKAR2A: a regulatory subunit of cAMP-regulated protein kinase. The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules. Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.

Protein type: Protein kinase, regulatory subunit

Chromosomal Location of Human Ortholog: 3p21.3-p21.2

Cellular Component: centrosome; focal adhesion; membrane; cytoplasm; plasma membrane; cytosol; cAMP-dependent protein kinase complex; AMP-activated protein kinase complex

Molecular Function: protein binding; ubiquitin protein ligase binding; cAMP-dependent protein kinase inhibitor activity; cAMP-dependent protein kinase regulator activity; cAMP binding

Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; phospholipase C activation; water transport; activation of protein kinase A; energy reserve metabolic process; renal water homeostasis; innate immune response; blood coagulation; signal transduction; regulation of insulin secretion; transmembrane transport