Mouse LDLRAP1 Monoclonal Antibody | anti-LDLRAP1 antibody

LDLRAP1 antibody

Cat. Num. AAA838161

• Gene Names: LDLRAP1; ARH; ARH1; ARH2; FHCB1; FHCB2
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: LDLRAP1 antibody was purified by affinity chromatography.
• Synonyms: LDLRAP1; Monoclonal Antibody; LDLRAP1 antibody; Monoclonal LDLRAP1; Anti-LDLRAP1; Low Density Lipoprotein Receptor Adaptor Protein 1; ARH; ARH1; ARH2; DKFZp586D0624; FHCB1; FHCB2; MGC34705; LDLRAP 1; LDLRAP-1; anti-LDLRAP1 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 5A12
• Purity/Purification: LDLRAP1 antibody was purified by affinity chromatography.
• Form/Format: Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 500 ug-1 mg/ml (varies by lot)
• Sequence Length: 308

• Applicable Applications for anti-LDLRAP1 antibody: Flow Cytometry (FC/FACS), Western Blot (WB)
• Application Notes: FC: 1:100; WB: 1:2000
• Biological Significance: LDLRAP1 is an adapter protein (clathrin-associated sorting protein (CLASP)) required for efficient endocytosis of the LDL receptor (LDLR) in polarized cells such as hepatocytes and lymphocytes, but not in non-polarized cells (fibroblasts). LDLRAP1 may be required for LDL binding and internalization but not for receptor clustering in coated pits. LDLRAP1 may facilitate the endocytocis of LDLR and LDLR-LDL complexes from coated pits by stabilizing the interaction between the receptor and the structural components of the pits.
• Cross-Reactivity: Human
• Immunogen: LDLRAP1 antibody was raised in mouse using a full length recombinant protein of human LDLRAP1 (NP_056442) produced in HEK293T cells, as the immunogen.
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant LDLRAP1 protein (Right) or empty vector (Left) detected with LDLRAP1 antibody)

Related Product Information for anti-LDLRAP1 antibody

Mouse monoclonal LDLRAP1 antibody

Product Categories/Family for anti-LDLRAP1 antibody

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 132626790
• NCBI GeneID: 26119
• NCBI Accession #: NP_056442.2
• NCBI GenBank Nucleotide #: NM_015627.2
• Molecular Weight: 33,885 Da
• NCBI Official Full Name: low density lipoprotein receptor adapter protein 1
• NCBI Official Synonym Full Names: low density lipoprotein receptor adaptor protein 1
• NCBI Official Symbol: LDLRAP1
• NCBI Official Synonym Symbols: ARH; ARH1; ARH2; FHCB1; FHCB2
• NCBI Protein Information: low density lipoprotein receptor adapter protein 1
• UniProt Protein Name: Low density lipoprotein receptor adapter protein 1
• Protein Family: Low density lipoprotein receptor adapter protein
• UniProt Gene Name: LDLRAP1
• UniProt Synonym Gene Names: ARH
• UniProt Entry Name: ARH_HUMAN

NCBI Description

The protein encoded by this gene is a cytosolic protein which contains a phosphotyrosine binding (PTD) domain. The PTD domain has been found to interact with the cytoplasmic tail of the LDL receptor. Mutations in this gene lead to LDL receptor malfunction and cause the disorder autosomal recessive hypercholesterolaemia. [provided by RefSeq, Jul 2008]

Uniprot Description

LDLRAP1: Adapter protein (clathrin-associated sorting protein (CLASP)) required for efficient endocytosis of the LDL receptor (LDLR) in polarized cells such as hepatocytes and lymphocytes, but not in non-polarized cells (fibroblasts). May be required for LDL binding and internalization but not for receptor clustering in coated pits. May facilitate the endocytocis of LDLR and LDLR-LDL complexes from coated pits by stabilizing the interaction between the receptor and the structural components of the pits. May also be involved in the internalization of other LDLR family members. Binds to phosphoinositides, which regulate clathrin bud assembly at the cell surface. Defects in LDLRAP1 are the cause of autosomal recessive hypercholesterolemia (ARH). ARH is a disorder caused by defective internalization of LDL receptors (LDLR) in the liver. ARH has the clinical features of familial hypercholesterolemia (FH) homozygotes, including severely elevated plasma LDL cholesterol, tuberous and tendon xanthomata, and premature atherosclerosis. LDL receptor (LDLR) activity measured in skin fibroblasts is normal, as the LDL binding ability.

Chromosomal Location of Human Ortholog: 1p36.11

Cellular Component: recycling endosome; internal side of plasma membrane; AP-1 adaptor complex; axon; early endosome; basal plasma membrane; neurofilament; AP-2 adaptor complex; cytosol

Molecular Function: clathrin binding; phosphatidylinositol-4,5-bisphosphate binding; protein binding; receptor signaling complex scaffold activity; low-density lipoprotein receptor binding; phosphotyrosine binding; beta-amyloid binding

Biological Process: cholesterol metabolic process; receptor-mediated endocytosis; cholesterol homeostasis; positive regulation of signal transduction; transport; amyloid precursor protein metabolic process; regulation of protein binding; receptor internalization; positive regulation of receptor-mediated endocytosis

Disease: Hypercholesterolemia, Autosomal Recessive