Rabbit DNA PK Polyclonal Antibody | anti-DNA antibody

DNA PK antibody

• Gene Names: PRKDC; HYRC; p350; DNAPK; DNPK1; HYRC1; IMD26; XRCC7; DNA-PKcs
• Applications: Western Blot
• Purity: DNA PK antibody was purified by immunoaffinity purification.
• Synonyms: DNA PK; Polyclonal Antibody; DNA PK antibody; Polyclonal DNA PK; Anti-DNA PK; DNA-PK; DNA dependent protein kinase; anti-DNA antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: DNA PK antibody was purified by immunoaffinity purification.
• Form/Format: Supplied in PBS buffer, pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
• Concentration: 500 ug/ml (varies by lot)
• Sequence Length: 930

• Applicable Applications for anti-DNA antibody: Western Blot (WB)
• Application Notes: WB: 0.5-4 ug/ml
• Biological Significance: DNA PK is made up of 3 subunits, 2 Ku proteins and a large catalytic subunit (DNA PKcs). DNA PKcs falls into the phosphatidyl inositol 3 kinase superfamily although it does have serine/threonine protein kinase activity. This subunit migrates on SDS PAGE gels as an intact and smaller processed form. In vitro, DNA PK phosphorylates several transcription factors and other DNA binding proteins and is believed to play a role in DNA damage recognition, repair and transcription. There is evidence to suggest that DNA PKcs may be a critical target for proteolysis by a caspase in apoptosis.
• Cross-Reactivity: Human, Mouse, Rat, Hamster
• Immunogen: DNA PK antibody was raised in Rabbit using synthetic peptide corresponding to residues surrounding amino acid 4088 of human DNA-PK as the immunogen.
• Preparation and Storage: Store at -20 degree C. For long-term storage, aliquot and freeze at -70 degree C. Avoid repeated freeze/thaw cycles.

Related Product Information for anti-DNA antibody

Rabbit polyclonal DNA PK antibody

Product Categories/Family for anti-DNA antibody

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 1017757
• NCBI GeneID: 5591
• UniProt Accession #: P78527; P78528; Q13327; Q13337; Q14175; Q59H99; Q7Z611; Q96SE6; Q9UME3
• Molecular Weight: 465,501 Da
• NCBI Official Full Name: DNA-PK, partial
• NCBI Official Synonym Full Names: protein kinase, DNA-activated, catalytic polypeptide
• NCBI Official Symbol: PRKDC
• NCBI Official Synonym Symbols: HYRC; p350; DNAPK; DNPK1; HYRC1; IMD26; XRCC7; DNA-PKcs
• NCBI Protein Information: DNA-dependent protein kinase catalytic subunit
• UniProt Protein Name: DNA-dependent protein kinase catalytic subunit
• UniProt Gene Name: PRKDC
• UniProt Synonym Gene Names: HYRC; HYRC1; DNA-PK catalytic subunit; DNA-PKcs
• UniProt Entry Name: PRKDC_HUMAN

NCBI Description

This gene encodes the catalytic subunit of the DNA-dependent protein kinase (DNA-PK). It functions with the Ku70/Ku80 heterodimer protein in DNA double strand break repair and recombination. The protein encoded is a member of the PI3/PI4-kinase family.[provided by RefSeq, Jul 2010]

Uniprot Description

DNAPK: an atypical protein kinase of the PIKK family. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. and modulation of transcription. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Defects cause severe combined immune deficiency (SCID) which is characterized by a lack of mature functional lymphocytes and a high susceptibility to lethal opportunistic infections. Required for repair of radiation-induced dsDNA breaks. Loss of function in mice or horses leads to the SCID (severe combined immune deficiency) phenotype due to failure of immunoglobulin rearrangement. Target of mutation in mismatch repair-deficient colorectal cancer. Inhibitor: KU-7059. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, atypical; DNA repair, damage; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Nucleolus; ATYPICAL group; PIKK family; DNAPK subfamily

Chromosomal Location of Human Ortholog: 8q11

Cellular Component: nucleoplasm; transcription factor complex; membrane; nucleolus; DNA-dependent protein kinase complex; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; enzyme binding; DNA binding; DNA-dependent protein kinase activity; transcription factor binding; ATP binding; protein kinase activity

Biological Process: positive regulation of apoptosis; heart development; germ cell programmed cell death; rhythmic process; T cell differentiation in the thymus; double-strand break repair via nonhomologous end joining; negative regulation of protein amino acid phosphorylation; double-strand break repair; positive regulation of interferon type I production; response to gamma radiation; telomere maintenance; pro-B cell differentiation; somitogenesis; immunoglobulin V(D)J recombination; protein destabilization; B cell lineage commitment; protein modification process; DNA repair; regulation of circadian rhythm; double-strand break repair via homologous recombination; peptidyl-serine phosphorylation; T cell lineage commitment; DNA damage response, signal transduction resulting in induction of apoptosis; cellular response to insulin stimulus; innate immune response; T cell receptor V(D)J recombination; positive regulation of transcription from RNA polymerase II promoter; brain development

Disease: Immunodeficiency 26 With Or Without Neurologic Abnormalities