Rabbit Cytokeratin 20 Polyclonal Antibody | anti-KRT20 antibody

Cytokeratin 20 antibody

Cat. Num. AAA835335

• Applications: Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
• Purity: Cytokeratin 20 antibody was purified by antigen-affinity chromatography
• Synonyms: Cytokeratin 20; Polyclonal Antibody; Cytokeratin 20 antibody; Polyclonal Cytokeratin 20; Anti-Cytokeratin 20; MGC35423; cytokeratin-20; K20; KRT21; CK-20; KRT20; CK20; Cytokeratin -20; keratin-20; CD20; anti-KRT20 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Cytokeratin 20 antibody was purified by antigen-affinity chromatography
• Form/Format: Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 424

• Applicable Applications for anti-KRT20 antibody: Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IF:1:100-1:200; ICC:1:100-1:200; IHC-P: 1:100-1:500; WB: 1:500-1:3000
• Biological Significance: The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21.
• Cross-Reactivity: Human
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Immunofluorescence (IF)

(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Cytokeratin 20 antibody at 1:200 dilution.)

Western Blot (WB)

(Western blot analysis of 30 ug of whole cell lysate (A: Hela) using a 10 % SDS PAGE gel and Cytokeratin 20 antibody at a dilution of 1:3000)

Immunohistochemistry (IHC)

(Immunohistochemical staining of paraffin-embedded HT29 xenograft using Cytokeratin 20 antibody at a dilution of 1:100)

Related Product Information for anti-KRT20 antibody

Affinity purified Rabbit polyclonal Cytokeratin 20 antibody

Product Categories/Family for anti-KRT20 antibody

Cell Biology; Purified Polyclonal Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 402645
• UniProt Accession #: P35900; B2R6W7
• Molecular Weight: 49 kDa
• NCBI Official Full Name: cytokeratin 20
• UniProt Protein Name: Keratin, type I cytoskeletal 20
• Protein Family: Keratin
• UniProt Gene Name: KRT20
• UniProt Synonym Gene Names: CK-20; K20
• UniProt Entry Name: K1C20_HUMAN

Uniprot Description

K20: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: cytoplasm; intermediate filament

Molecular Function: protein binding; structural constituent of cytoskeleton

Biological Process: regulation of protein secretion; apoptosis; cellular response to stress; intermediate filament organization