Mouse NUDT6 Monoclonal Antibody | anti-NUDT6 antibody

NUDT6 antibody

Cat. Num. AAA833683

• Gene Names: NUDT6; GFG1; GFG-1; ASFGF2; FGF-AS; FGF2AS
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot
• Purity: NUDT6 antibody was purified by affinity chromatography.
• Synonyms: NUDT6; Monoclonal Antibody; NUDT6 antibody; Monoclonal NUDT6; Anti-NUDT6; Nudix type motif 6; Nucleoside diphosphate linked moiety X; FGF2AS; gfg; Antisense basic fibroblast growth factor; Nudix motif 6; ASFGF2; bFGF; NUDT 6; NUDT-6; anti-NUDT6 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 10B4
• Purity/Purification: NUDT6 antibody was purified by affinity chromatography.
• Form/Format: Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 500 ug-1 mg/ml (varies by lot)
• Sequence Length: 316

• Applicable Applications for anti-NUDT6 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB)
• Application Notes: FC: 1:100; IF: 1:100; WB: 1:200-500
• Biological Significance: FGF2 is a highly conserved, multifunctional heparin-binding growth factor involved in neuroectoderm development, angiogenesis, and wound healing. Elevated levels of FGF2 are associated with proliferation of smooth muscle in atherosclerosis and with proliferation of tumors. The FGF2 antisense gene, NUDT6, may regulate FGF2 expression.
• Cross-Reactivity: Human, Monkey
• Immunogen: NUDT6 antibody was raised in mouse using a full length recombinant protein of human NUDT6 (NP_009014) produced in HEK293T cells, as the immunogen.
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of HEK293T cells transfected with either recombinant NUDT6 protein (red) or empty vector (blue) stained using NUDT6 antibody)

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant NUDT6 protein (Right) or empty vector (Left) detected with NUDT6 antibody)

Immunofluorescence (IF)

(Immunofluorescent staining of COS7 cells transiently transfected with recombinant NUDT6 protein using NUDT6 antibody)

Related Product Information for anti-NUDT6 antibody

Mouse monoclonal NUDT6 antibody

Product Categories/Family for anti-NUDT6 antibody

DNA & RNA

NCBI and Uniprot Product Information

• NCBI GI #: 20149583
• NCBI GeneID: 11162
• NCBI Accession #: NP_009014
• NCBI GenBank Nucleotide #: NM_007083.4
• UniProt Accession #: P53370; O95097; Q9UQD9; A8K756
• Molecular Weight: 17,082 Da
• NCBI Official Full Name: nucleoside diphosphate-linked moiety X motif 6 isoform a
• NCBI Official Synonym Full Names: nudix (nucleoside diphosphate linked moiety X)-type motif 6
• NCBI Official Symbol: NUDT6
• NCBI Official Synonym Symbols: GFG1; GFG-1; ASFGF2; FGF-AS; FGF2AS
• NCBI Protein Information: nucleoside diphosphate-linked moiety X motif 6
• UniProt Protein Name: Nucleoside diphosphate-linked moiety X motif 6
• Protein Family: Nudix hydrolase
• UniProt Gene Name: NUDT6
• UniProt Synonym Gene Names: FGF2AS; Nudix motif 6
• UniProt Entry Name: NUDT6_HUMAN

NCBI Description

This gene overlaps and lies on the opposite strand from FGF2 gene, and is thought to be the FGF2 antisense gene. The two genes are independently transcribed, and their expression shows an inverse relationship, suggesting that this antisense transcript may regulate FGF2 expression. This gene has also been shown to have hormone-regulatory and antiproliferative actions in the pituitary that are independent of FGF2 expression. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

NUDT6: May contribute to the regulation of cell proliferation. Belongs to the Nudix hydrolase family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 4q26

Cellular Component: mitochondrion; nucleus

Molecular Function: growth factor activity; hydrolase activity

Biological Process: metabolic process