Immunofluorescence (IF)
(Immunocytochemistry of fixed and permeabilized MDCK cells using biotinylated alpha Tubulin antibody followed by Streptavidin FITC.)
Mouse alpha Tubulin Monoclonal Antibody
alpha Tubulin antibody (biotin)
Applications
Flow Cytometry, Functional Assay
Synonyms
alpha Tubulin; Monoclonal Antibody; alpha Tubulin antibody (biotin); Monoclonal alpha Tubulin; Anti- alpha Tubulin; alpha-Tubulin; a-Tubulin; anti-alpha Tubulin antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1 kappa
Clone Number
DM1A
Form/Format
Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration
500 ug/ml (varies by lot)
Applicable Applications for anti-alpha Tubulin antibody
Flow Cytometry (FC/FACS)
Application Notes
FC: <= 0.5 ug/test
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
Biological Significance
The monoclonal antibody DM1a recognizes the 50kDa cytoskeletal protein alpha tubulin in a variety of species. Tubulin, the major component of microtubules, is a dimeric protein consisting of an alpha and beta subunit. Tubulin is a GTP-binding protein that can be modified by phosphorylation and acetylation resulting in assembly or disassembly. The dynamic nature of microtubules is most evident in the mitotic apparatus. The DM1a antibody recognizes the C-terminal end of the alpha tubulin isoform.
Tag/Conjugate
Biotin
Preparation and Storage
Store at 2-8 degree C. Do not freeze. Protect from light.
Immunofluorescence (IF)
(Immunocytochemistry of fixed and permeabilized MDCK cells using biotinylated alpha Tubulin antibody followed by Streptavidin FITC.)
Immunofluorescence (IF)
(Immunocytochemistry of fixed and permeabilized MDCK cells using biotinylated alpha Tubulin antibody followed by Streptavidin FITC.)
Related Product Information for anti-alpha Tubulin antibody
Mouse monoclonal alpha Tubulin antibody (biotin)
Product Categories/Family for anti-alpha Tubulin antibody
Similar Products
Product Notes
The alpha Tubulin (Catalog #AAA833243) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's alpha Tubulin can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC: <= 0.5 ug/test A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Researchers should empirically determine the suitability of the alpha Tubulin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "alpha Tubulin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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