Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant DYNC1LI1 protein (Right) or empty vector (Left) detected with DYNC1LI1 antibody)
Mouse DYNC1LI1 Monoclonal Antibody | anti-DYNC1LI1 antibody
DYNC1LI1 antibody
Gene Names
DYNC1LI1; LIC1; DNCLI1
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot
Purity
DYNC1LI1 antibody was purified by affinity chromatography.
Synonyms
DYNC1LI1; Monoclonal Antibody; DYNC1LI1 antibody; Monoclonal DYNC1LI1; Anti-DYNC1LI1; Dynein cytoplasmi 1c light intermediate chain 1; Dynein light chain A; Dynein light intermediate chain 1 cytosolic; DLC-A; DNCLI1; DYNCLI 1; DYNCLI-1; anti-DYNC1LI1 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
1H4
Purity/Purification
DYNC1LI1 antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
500 ug-1 mg/ml (varies by lot)
Sequence Length
523
Applicable Applications for anti-DYNC1LI1 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB)
Application Notes
FC: 1:100
IF: 1:100
WB: 1:2000
IF: 1:100
WB: 1:2000
Biological Significance
DYNC1LI1 acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that arethought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmicdynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. Mayplay a role in binding dynein to membranous organelles or chromosomes. Probably involved in the microtubule-dependent transport of pericentrin. Is required for progress throuh the spindle assembly checkpoint.
Cross-Reactivity
Human
Immunogen
DYNC1LI1 antibody was raised in mouse using a full length recombinant protein of human DYNC1LI1 (NP_057225) produced in HEK293T cells, as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant DYNC1LI1 protein (Right) or empty vector (Left) detected with DYNC1LI1 antibody)
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant DYNC1LI1 protein (Right) or empty vector (Left) detected with DYNC1LI1 antibody)
Immunofluorescence (IF)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant DYNC1LI1 protein using DYNC1LI1 antibody)
Immunofluorescence (IF)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant DYNC1LI1 protein using DYNC1LI1 antibody)
Related Product Information for anti-DYNC1LI1 antibody
Mouse monoclonal DYNC1LI1 antibody
Product Categories/Family for anti-DYNC1LI1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
56,579 Da
NCBI Official Full Name
cytoplasmic dynein 1 light intermediate chain 1
NCBI Official Synonym Full Names
dynein, cytoplasmic 1, light intermediate chain 1
NCBI Official Symbol
DYNC1LI1
NCBI Official Synonym Symbols
LIC1; DNCLI1
NCBI Protein Information
cytoplasmic dynein 1 light intermediate chain 1
UniProt Protein Name
Cytoplasmic dynein 1 light intermediate chain 1
Protein Family
UniProt Gene Name
DYNC1LI1
UniProt Synonym Gene Names
DNCLI1; LIC1; DLC-A
UniProt Entry Name
DC1L1_HUMAN
Uniprot Description
DNCLI1: a cytoskeletal motor protein that is a subunit of cytoplasmic dynein. Possesses GTPase activity. Its function is regulated by phosphorylation. Cytoplasmic dynein is a minus-end-directed, microtubule-dependent motor composed of two heavy chains, three intermediate chains, and a family of light chains.
Protein type: Cell cycle regulation; Microtubule-binding; Motility/polarity/chemotaxis; Motor; Cytoskeletal
Chromosomal Location of Human Ortholog: 3p22.3
Cellular Component: kinetochore; spindle pole; centrosome; microtubule; membrane; cytoplasmic dynein complex; cytoplasm; plasma membrane
Molecular Function: microtubule motor activity; ATP binding
Biological Process: mitosis; viral reproduction; transport; metabolic process; cell division; microtubule-based movement
Research Articles on DYNC1LI1
Similar Products
Product Notes
The DYNC1LI1 dync1li1 (Catalog #AAA830756) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's DYNC1LI1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB). FC: 1:100 IF: 1:100 WB: 1:2000. Researchers should empirically determine the suitability of the DYNC1LI1 dync1li1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DYNC1LI1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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