Western Blot (WB)
(Western blot analysis of EIF2B expression in mouse thymus (A), rat kidney (B) whole cell lysates.)
Rabbit EIF2B Polyclonal Antibody | anti-EIF2B antibody
Anti-EIF2B Antibody
IHC: 1:50-1:200
IP: 1:20-1:100
Western Blot (WB)
(Western blot analysis of EIF2B expression in mouse thymus (A), rat kidney (B) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of EIF2B expression in mouse thymus (A), rat kidney (B) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of EIF2B staining in human stomach formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of EIF2B staining in human stomach formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the beta subunit of eukaryotic initiation factor-2B (EIF2B). EIF2B is involved in protein synthesis and exchanges GDP and GTP for its activation and deactivation. [provided by RefSeq, Aug 2011]
Uniprot Description
eIF2B-beta: eukaryotic translation initiation factor 2B, subunit 5. A translational regulatory protein that functions in the early steps of protein synthesis by catalyzing the exchange of eukaryotic initiation factor 2-bound GDP for GTP. Mutation in EIF2B5 causes childhood ataxia with central nervous system hypomyelination/ vanishing white matter leukodystrophy.
Protein type: Cell development/differentiation; RNA-binding; Translation; GEFs; GEFs, misc.
Chromosomal Location of Human Ortholog: 14q24.3
Cellular Component: eukaryotic translation initiation factor 2B complex; cytoplasm; cytosol
Molecular Function: protein binding; GTP binding; guanyl-nucleotide exchange factor activity; translation initiation factor activity; S-methyl-5-thioribose-1-phosphate isomerase activity; ATP binding
Biological Process: myelination; response to peptide hormone stimulus; central nervous system development; translation; cellular response to stimulus; cellular protein metabolic process; ovarian follicle development; methionine salvage; response to heat; translational initiation; response to glucose stimulus; gene expression; oligodendrocyte development; regulation of translational initiation; positive regulation of GTPase activity
Disease: Leukoencephalopathy With Vanishing White Matter
Research Articles on EIF2B
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Product Notes
The EIF2B eif2b2 (Catalog #AAA8306554) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-EIF2B Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's EIF2B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP). WB: 1:500-1:2000 IHC: 1:50-1:200 IP: 1:20-1:100. Researchers should empirically determine the suitability of the EIF2B eif2b2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EIF2B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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