Rabbit ABCE1 Polyclonal Antibody | anti-ABCE1 antibody

Anti-ABCE1 Antibody

Cat. Num. AAA8305933

• Gene Names: ABCE1; RLI; OABP; RLI1; ABC38; RNS4I; RNASEL1; RNASELI
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence, Immunocytochemistry
• Purity: Purified by Immunogen Affinity Chromatography
• Synonyms: ABCE1; Polyclonal Antibody; Anti-ABCE1 Antibody; RLI; RNASEL1; RNASELI; RNS4I; ATP-binding cassette sub-family E member 1; 2'-5'-oligoadenylate-binding protein; HuHP68; RNase L inhibitor; Ribonuclease 4 inhibitor; anti-ABCE1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Recognizes endogenous levels of ABCE1 protein.
• Purity/Purification: Purified by Immunogen Affinity Chromatography
• Form/Format: Liquid in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3, 30% Glycerol, and 0.01% Sodium Azide.

• Applicable Applications for anti-ABCE1 antibody: Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
• Application Notes: WB: 1:500-1:2000; IF/ICC: 1:50-1:200
• Immunogen: Recombinant fusion protein of human ABCE1. The exact sequence is proprietary.
• Preparation and Storage: Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of ABCE1 expression in Jurkat (A), HeLa (B), mouse liver (C) whole cell lysates.)

Immunofluorescence (IF)/Immunocytochemistry (ICC)

(Immunofluorescent analysis of ABCE1 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 488-conjugated secondary antibody (green) in PBS at room temperature in the dark.)

Related Product Information for anti-ABCE1 antibody

Rabbit polyclonal antibody to ABCE1

NCBI and Uniprot Product Information

• NCBI GI #: 108773784
• NCBI GeneID: 6059
• NCBI Accession #: NP_001035809.1
• NCBI GenBank Nucleotide #: NM_001040876.1
• UniProt Accession #: P61221; P61222
• Molecular Weight: 67,314 Da
• NCBI Official Full Name: ATP-binding cassette sub-family E member 1
• NCBI Official Synonym Full Names: ATP binding cassette subfamily E member 1
• NCBI Official Symbol: ABCE1
• NCBI Official Synonym Symbols: RLI; OABP; RLI1; ABC38; RNS4I; RNASEL1; RNASELI
• NCBI Protein Information: ATP-binding cassette sub-family E member 1
• UniProt Protein Name: ATP-binding cassette sub-family E member 1
• Protein Family: ABC transporter E family
• UniProt Gene Name: ABCE1
• UniProt Synonym Gene Names: RLI; RNASEL1; RNASELI; RNS4I; RNS4I

NCBI Description

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the OABP subfamily. Alternatively referred to as the RNase L inhibitor, this protein functions to block the activity of ribonuclease L. Activation of ribonuclease L leads to inhibition of protein synthesis in the 2-5A/RNase L system, the central pathway for viral interferon action. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ABCE1: Antagonizes the binding of 2-5A (5'-phosphorylated 2',5'-linked oligoadenylates) by RNase L through direct interaction with RNase L and therefore inhibits its endoribonuclease activity. May play a central role in the regulation of mRNA turnover. Antagonizes the anti-viral effect of the interferon-regulated 2-5A/RNase L pathway. May act as a chaperone for post-translational events during HIV-1 capsid assembly. Belongs to the ABC transporter superfamily. ABCE family.

Chromosomal Location of Human Ortholog: 4q31.21

Cellular Component: cytoplasm; eukaryotic translation initiation factor 3 complex; membrane; mitochondrial matrix; mitochondrion

Molecular Function: ATP binding; iron ion binding; protein binding; ribosomal small subunit binding

Biological Process: ribosome export from nucleus; translational initiation; translational termination; transmembrane transport