Western Blot (WB)
(Western blot analysis of NARFL expression in MCF7 (A), H1792 (B) whole cell lysates.)
Rabbit anti-Human NARFL Polyclonal Antibody | anti-NARFL antibody
Anti-NARFL Antibody
Gene Names
CIAO3; PRN; HPRN; IOP1; NAR1; LET1L; NARFL
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Purified by immunogen affinity chromatography.
Synonyms
NARFL; Polyclonal Antibody; Anti-NARFL Antibody; PRN; Cytosolic Fe-S cluster assembly factor NARFL; Iron-only hydrogenase-like protein 1; IOP1; Nuclear prelamin A recognition factor-like protein; Protein related to Narf; anti-NARFL antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of NARFL protein.
Purity/Purification
Purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3, 30% Glycerol, and 0.01% Sodium Azide.
Sequence Length
374
Applicable Applications for anti-NARFL antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:100
IHC: 1:50-1:100
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human NARFL.
Preparation and Storage
Upon delivery aliquot and store at-20 degree C for one year. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of NARFL expression in MCF7 (A), H1792 (B) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of NARFL expression in MCF7 (A), H1792 (B) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of NARFL staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of NARFL staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Related Product Information for anti-NARFL antibody
Rabbit polyclonal antibody to NARFL
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
26,135 Da
NCBI Official Full Name
cytosolic iron-sulfur assembly component 3 isoform 2
NCBI Official Synonym Full Names
cytosolic iron-sulfur assembly component 3
NCBI Official Symbol
CIAO3
NCBI Official Synonym Symbols
PRN; HPRN; IOP1; NAR1; LET1L; NARFL
NCBI Protein Information
cytosolic iron-sulfur assembly component 3
UniProt Protein Name
Cytosolic iron-sulfur assembly component 3
Protein Family
UniProt Gene Name
CIAO3
UniProt Synonym Gene Names
IOP11 PublicationManual assertion based on opinion iniRef.8
Uniprot Description
Component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into extramitochondrial Fe/S proteins. Seems to negatively regulate the level of HIF1A expression, although this effect could be indirect.
Research Articles on NARFL
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Product Notes
The NARFL ciao3 (Catalog #AAA8304028) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-NARFL Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NARFL can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:1000 IHC: 1:50-1:100. Researchers should empirically determine the suitability of the NARFL ciao3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NARFL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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