Western Blot (WB)
(Western Blot showing CHUK antibody used against Raji (1), Jurkat (2), THP-1 (3) and K562 (4) cell lysate.)
Mouse CHUK Monoclonal Antibody | anti-CHUK antibody
CHUK antibody
IF: 1:200-1:1000
WB: 1:500-1:2000
Western Blot (WB)
(Western Blot showing CHUK antibody used against Raji (1), Jurkat (2), THP-1 (3) and K562 (4) cell lysate.)
Western Blot (WB)
(Western Blot showing CHUK antibody used against Raji (1), Jurkat (2), THP-1 (3) and K562 (4) cell lysate.)
Immunofluorescence (IF)
(Immunofluorescence analysis of NIH/3T3 cells using CHUK antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
Immunofluorescence (IF)
(Immunofluorescence analysis of NIH/3T3 cells using CHUK antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. [provided by RefSeq, Jul 2008]
Uniprot Description
IKKA: a kinase of the IKK family. Phosphorylates inhibitors of NF-kappa-B, leading to their dissociation from NF-kappa-B and ultimately to their degradation. Phosphorylated and activated downstream of growth factor receptors, IL1R, and TNFR by NAK, IRAK and NIK, respectively. Preferentially found as a heterodimer with IKK-beta but also as an homodimer. Directly interacts with IKK-gamma/NEMO and TRPC4AP. Heterodimers form the active complex. The tripartite complex can also bind to MAP3K14/NIK, MEKK1, IKAP and IKB-alpha-p65-p50 complex. Inhibitors are under development to treat arthritis, inflammation, and apoptotic aspects of cancer. Aspirin (sodium salicylate) selectively binds IKK and reduces inflammation. Misexpression and inhibitors show involvement in insulin sensitization in obese rodents. Mutations in IKK-gamma are found in several immune deficiencies: hyper IgM syndrome, incontinentia pigmenti [embryonic lethal in males, defects in skin, hair, teeth in females] and hypohidrotic ectodermal dysplasia; recurrent infections and defects in teeth, hair, and sweat glands]. Inhibitors: CHS 828, NBD peptide, BMS-345541.
Protein type: EC 2.7.11.10; Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; Kinase, protein; Other group; IKK family
Chromosomal Location of Human Ortholog: 10q24-q25
Cellular Component: nucleoplasm; internal side of plasma membrane; intracellular membrane-bound organelle; cytoplasm; IkappaB kinase complex; cytosol
Molecular Function: protein binding; protein homodimerization activity; IkappaB kinase activity; protein heterodimerization activity; ATP binding; protein kinase activity
Biological Process: skeletal muscle contraction; lactation; I-kappaB kinase/NF-kappaB cascade; nerve growth factor receptor signaling pathway; response to toxin; response to lipopolysaccharide; toll-like receptor 3 signaling pathway; osteoclast differentiation; protein amino acid phosphorylation; T cell receptor signaling pathway; Rho protein signal transduction; toll-like receptor 10 signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 5 signaling pathway; positive regulation of interferon type I production; morphogenesis of an epithelial sheet; inflammatory response; toll-like receptor 4 signaling pathway; response to drug; epidermal growth factor receptor signaling pathway; anatomical structure morphogenesis; positive regulation of I-kappaB kinase/NF-kappaB cascade; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; response to virus; MyD88-independent toll-like receptor signaling pathway; mammary gland epithelial cell proliferation; response to amino acid stimulus; odontogenesis of dentine-containing teeth; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; response to hydroperoxide; toll-like receptor signaling pathway; striated muscle cell differentiation; innate immune response; immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; I-kappaB phosphorylation
Disease: Cocoon Syndrome
Research Articles on CHUK
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Product Notes
The CHUK chuk (Catalog #AAA830339) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CHUK can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunofluorescence (IF), Western Blot (WB). ELISA: 1:10,000 IF: 1:200-1:1000 WB: 1:500-1:2000. Researchers should empirically determine the suitability of the CHUK chuk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CHUK, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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