CTU1 sirna

CTU1 siRNA (Mouse)

Cat. Num. AAA829899

• Gene Names: Ctu1; Atpbd3; BC005752
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CTU1; CTU1 siRNA (Mouse); ATPBD3; NCS6; Cytoplasmic tRNA 2-thiolation protein 1; ATP-binding domain-containing protein 3; Cytoplasmic tRNA adenylyltransferase 1; CTU1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: CTU1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 420

• Applicable Applications for CTU1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CTU1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CTU1 sirna

siRNA to inhibit CTU1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 21704170
• NCBI GeneID: 233189
• NCBI Accession #: NP_663557.1
• NCBI GenBank Nucleotide #: NM_145582.1
• UniProt Accession #: Q99J10
• Molecular Weight: 43,823 Da
• NCBI Official Full Name: cytoplasmic tRNA 2-thiolation protein 1
• NCBI Official Synonym Full Names: cytosolic thiouridylase subunit 1 homolog (S. pombe)
• NCBI Official Symbol: Ctu1
• NCBI Official Synonym Symbols: Atpbd3; BC005752
• NCBI Protein Information: cytoplasmic tRNA 2-thiolation protein 1
• UniProt Protein Name: Cytoplasmic tRNA 2-thiolation protein 1
• Protein Family: CTU1/ATPBD3 family protein
• UniProt Gene Name: Ctu1
• UniProt Synonym Gene Names: Atpbd3; Ncs6
• UniProt Entry Name: CTU1_MOUSE

Uniprot Description

ATPBD3: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position. Belongs to the TtcA family. CTU1/NCS6/ATPBD3 subfamily.

Protein type: EC 2.7.7.-

Cellular Component: mitochondrion; cytoplasm

Molecular Function: transferase activity; telomerase activity; tRNA guanylyltransferase activity; DNA primase activity; CTP:2,3-di-O-geranylgeranyl-sn-glycero-1-phosphate cytidyltransferase; RNA binding; DNA/RNA helicase activity; sulfate adenylyltransferase activity; RNA guanylyltransferase activity; mannose-phosphate guanylyltransferase activity; phospholactate guanylyltransferase activity; RNA helicase activity; tRNA binding

Biological Process: tRNA wobble uridine modification; tRNA processing