APTX sirna

APTX siRNA (Rat)

Cat. Num. AAA829802

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: APTX; APTX siRNA (Rat); Aprataxin; Forkhead-associated domain histidine triad-like protein; FHA-HIT; APTX sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: APTX siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 329

• Applicable Applications for APTX sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat APTX gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for APTX sirna

siRNA to inhibit APTX expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 22507312
• NCBI GeneID: 259271
• NCBI Accession #: NP_683687.1
• NCBI GenBank Nucleotide #: NM_148889.2
• UniProt Accession #: Q8K4H4
• Molecular Weight: 37,720 Da
• NCBI Official Full Name: aprataxin
• NCBI Official Synonym Full Names: aprataxin
• NCBI Official Symbol: Aptx
• NCBI Protein Information: aprataxin
• UniProt Protein Name: Aprataxin
• Protein Family: Aprataxin
• UniProt Gene Name: Aptx
• UniProt Synonym Gene Names: FHA-HIT
• UniProt Entry Name: APTX_RAT

NCBI Description

human homolog may play a role in DNA repair [RGD, Feb 2006]

Uniprot Description

APTX: DNA-binding protein involved in single-strand DNA break repair, double-strand DNA break repair and base excision repair. Resolves abortive DNA ligation intermediates formed either at base excision sites, or when DNA ligases attempt to repair non- ligatable breaks induced by reactive oxygen species. Catalyzes the release of adenylate groups covalently linked to 5'-phosphate termini, resulting in the production of 5'-phosphate termini that can be efficiently rejoined. Also able to hydrolyze adenosine 5'- monophosphoramidate (AMP-NH(2)) and diadenosine tetraphosphate (AppppA), but with lower catalytic activity. Defects in APTX are the cause of ataxia-oculomotor apraxia syndrome (AOA). AOA is an autosomal recessive syndrome characterized by early-onset cerebellar ataxia, oculomotor apraxia, early areflexia and late peripheral neuropathy. 13 isoforms of the human protein are produced by alternative splicing.

Protein type: C2H2-type zinc finger protein; DNA repair, damage; EC 3.-.-.-; Nucleolus; Phosphatase (non-protein); RNA-binding

Cellular Component: chromatin; nuclear chromatin; nucleolus; nucleoplasm; nucleus

Molecular Function: chromatin binding; damaged DNA binding; DNA 5'-adenosine monophosphate hydrolase activity; double-stranded DNA binding; double-stranded RNA binding; phosphoglycolate phosphatase activity; phosphoprotein binding; polynucleotide 3'-phosphatase activity; protein N-terminus binding

Biological Process: DNA ligation; double-strand break repair; regulation of protein stability; response to DNA damage stimulus; response to hydrogen peroxide; single strand break repair