RIN2 sirna

RIN2 siRNA (Human)

Cat. Num. AAA829683

• Gene Names: RIN2; MACS; RASSF4
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RIN2; RIN2 siRNA (Human); RASSF4; Ras and Rab interactor 2; Ras association domain family 4; Ras inhibitor JC265; Ras interaction/interference protein 2; RIN2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: RIN2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 944

• Applicable Applications for RIN2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RIN2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RIN2 sirna

siRNA to inhibit RIN2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 336088636
• NCBI GeneID: 54453
• NCBI Accession #: NP_001229510.1
• NCBI GenBank Nucleotide #: NM_001242581.1
• UniProt Accession #: Q8WYP3; Q00425; Q5TFT8; Q9BQL3; Q9H071
• Molecular Weight: 105,613 Da
• NCBI Official Full Name: ras and Rab interactor 2 isoform 1
• NCBI Official Synonym Full Names: Ras and Rab interactor 2
• NCBI Official Symbol: RIN2
• NCBI Official Synonym Symbols: MACS; RASSF4
• NCBI Protein Information: ras and Rab interactor 2
• UniProt Protein Name: Ras and Rab interactor 2
• Protein Family: Ras and Rab interactor
• UniProt Gene Name: RIN2
• UniProt Synonym Gene Names: RASSF4
• UniProt Entry Name: RIN2_HUMAN

NCBI Description

The RAB5 protein is a small GTPase involved in membrane trafficking in the early endocytic pathway. The protein encoded by this gene binds the GTP-bound form of the RAB5 protein preferentially over the GDP-bound form, and functions as a guanine nucleotide exchange factor for RAB5. The encoded protein is found primarily as a tetramer in the cytoplasm and does not bind other members of the RAB family. Mutations in this gene cause macrocephaly alopecia cutis laxa and scoliosis (MACS) syndrome, an elastic tissue disorder, as well as the related connective tissue disorder, RIN2 syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2011]

Uniprot Description

RIN2: Ras effector protein. May function as an upstream activator and/or downstream effector for RAB5B in endocytic pathway. May function as a guanine nucleotide exchange (GEF) of RAB5B, required for activating the RAB5 proteins by exchanging bound GDP for free GTP. Defects in RIN2 are the cause of MACS syndrome (MACS); also called macrocephaly alopecia cutis laxa and scoliosis syndrome. MACS is an autosomal-recessive inherited complex disorder of elastic tissue, characterized by sagging skin and occasionally by life-threatening visceral complications. Belongs to the RIN (Ras interaction/interference) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: GEFs, Rab; GEFs; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 20p11.22

Cellular Component: cytoplasm

Molecular Function: Rab guanyl-nucleotide exchange factor activity; GTPase activator activity

Biological Process: regulation of catalytic activity; small GTPase mediated signal transduction; endocytosis

Disease: Macs Syndrome