ACE2 sirna

ACE2 siRNA (Mouse)

Cat. Num. AAA829658

• Gene Names: Ace2; 2010305L05Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ACE2; ACE2 siRNA (Mouse); Angiotensin-converting enzyme 2; ACE-related carboxypeptidase; ACE2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ACE2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 805

• Applicable Applications for ACE2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ACE2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ACE2 sirna

siRNA to inhibit ACE2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 194473667
• NCBI GeneID: 70008
• NCBI Accession #: NP_001123985.1
• NCBI GenBank Nucleotide #: NM_001130513.1
• UniProt Accession #: Q8R0I0; Q99N70; Q99N71
• Molecular Weight: 40,340 Da
• NCBI Official Full Name: angiotensin-converting enzyme 2
• NCBI Official Synonym Full Names: angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
• NCBI Official Symbol: Ace2
• NCBI Official Synonym Symbols: 2010305L05Rik
• NCBI Protein Information: angiotensin-converting enzyme 2
• UniProt Protein Name: Angiotensin-converting enzyme 2
• Protein Family: Angiotensin-converting enzyme
• UniProt Gene Name: Ace2
• UniProt Entry Name: ACE2_MOUSE

Uniprot Description

ACE2: Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin- 13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses. Interacts with ITGB1. Interacts with SARS-CoV and HCoV- NL63 spike glycoprotein. Up-regulated in failing heart. Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells. Expressed in heart, kidney, testis, and gastrointestinal system. Activated by chloride and fluoride, but not bromide. Inhibited by MLN-4760, cFP_Leu, and EDTA, but not by the ACE inhibitors linosipril, captopril and enalaprilat. Belongs to the peptidase M2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; EC 3.4.17.23; Protease

Cellular Component: extracellular space; cell surface; membrane; cytoplasm; plasma membrane; integral to membrane; extracellular region

Molecular Function: peptidase activity; viral receptor activity; peptidyl-dipeptidase activity; carboxypeptidase activity; hydrolase activity; metallopeptidase activity; metal ion binding; endopeptidase activity; glycoprotein binding

Biological Process: virion attachment, binding of host cell surface receptor; negative regulation of smooth muscle cell proliferation; receptor biosynthetic process; proteolysis; angiotensin mediated drinking behavior; regulation of systemic arterial blood pressure by renin-angiotensin