TF sirna

TF siRNA (Rat)

Cat. Num. AAA829280

• Gene Names: Tf; Tfn; Trf
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TF; TF siRNA (Rat); Serotransferrin; Transferrin; Beta-1 metal-binding globulin; Liver regeneration-related protein LRRG03; Siderophilin; TF sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: TF siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 698

• Applicable Applications for TF sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat TF gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TF sirna

siRNA to inhibit TF expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 61556986
• NCBI GeneID: 24825
• NCBI Accession #: NP_001013128.1
• NCBI GenBank Nucleotide #: NM_001013110.1
• UniProt Accession #: P12346; Q63602; Q64628; Q64630; Q7TNX0
• Molecular Weight: 54,524 Da
• NCBI Official Full Name: serotransferrin
• NCBI Official Synonym Full Names: transferrin
• NCBI Official Symbol: Tf
• NCBI Official Synonym Symbols: Tfn; Trf
• NCBI Protein Information: serotransferrin
• UniProt Protein Name: Serotransferrin
• Protein Family: TFIIA-alpha and beta-like factor
• UniProt Gene Name: Tf
• UniProt Synonym Gene Names: Transferrin
• UniProt Entry Name: TRFE_RAT

NCBI Description

plays a role iin iron transport and homeostasis [RGD, Feb 2006]

Uniprot Description

TF: Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation. Defects in TF are the cause of atransferrinemia (ATRAF). Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia. Belongs to the transferrin family.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular space; cell surface; early endosome; cytoplasmic membrane-bound vesicle; coated pit; recycling endosome; endocytic vesicle; perinuclear region of cytoplasm; late endosome; apical plasma membrane; basal plasma membrane; basement membrane; endosome; vesicle

Molecular Function: GTP binding; ferric iron binding; ferric iron transmembrane transporter activity

Biological Process: positive regulation of myelination; cellular iron ion homeostasis; apoptosis; positive regulation of transcription, DNA-dependent; actin filament organization; osteoclast differentiation; response to organic cyclic substance; iron ion transport; activation of JNK activity; retinal homeostasis; small GTPase mediated signal transduction; response to lead ion; response to hypoxia; acute-phase response; positive regulation of bone resorption; ferric iron transport; positive regulation of phosphorylation