RND1 sirna

RND1 siRNA (Human)

Cat. Num. AAA829035

• Gene Names: RND1; ARHS; RHO6; RHOS
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RND1; RND1 siRNA (Human); RHO6; Rho-related GTP-binding protein Rho6; Rho family GTPase 1; Rnd1; RND1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: RND1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 232

• Applicable Applications for RND1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RND1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RND1 sirna

siRNA to inhibit RND1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 7657514
• NCBI GeneID: 27289
• NCBI Accession #: NP_055285.1
• NCBI GenBank Nucleotide #: NM_014470.3
• UniProt Accession #: Q92730; A8K9P7
• Molecular Weight: 26,056 Da
• NCBI Official Full Name: rho-related GTP-binding protein Rho6
• NCBI Official Synonym Full Names: Rho family GTPase 1
• NCBI Official Symbol: RND1
• NCBI Official Synonym Symbols: ARHS; RHO6; RHOS
• NCBI Protein Information: rho-related GTP-binding protein Rho6
• UniProt Protein Name: Rho-related GTP-binding protein Rho6
• Protein Family: Rho-related GTP-binding protein
• UniProt Gene Name: RND1
• UniProt Synonym Gene Names: RHO6
• UniProt Entry Name: RND1_HUMAN

NCBI Description

This gene encodes a protein that belongs to the Rho GTPase family. Members of this family regulate the organization of the actin cytoskeleton in response to extracellular growth factors. A similar protein in rat interacts with a microtubule regulator to control axon extension. [provided by RefSeq, Apr 2014]

Uniprot Description

RND1: Lacks intrinsic GTPase activity. Has a low affinity for GDP, and constitutively binds GTP. Controls rearrangements of the actin cytoskeleton. Induces the Rac-dependent neuritic process formation in part by disruption of the cortical actin filaments. Causes the formation of many neuritic processes from the cell body with disruption of the cortical actin filaments. Belongs to the small GTPase superfamily. Rho family.

Protein type: G protein, monomeric; G protein; G protein, monomeric, Rho

Chromosomal Location of Human Ortholog: 12q12

Cellular Component: adherens junction; cytoskeleton; plasma membrane; cytosol

Molecular Function: GTPase activity; protein binding; GTP binding; receptor binding

Biological Process: axon guidance; metabolic process; small GTPase mediated signal transduction; actin filament organization; negative regulation of cell adhesion; neuron remodeling