LHCGR sirna

LHCGR siRNA (Mouse)

Cat. Num. AAA828910

• Gene Names: Lhcgr; Lhr; LH-R; Gpcr19-rs1
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: LHCGR; LHCGR siRNA (Mouse); LHR; Lutropin-choriogonadotropic hormone receptor; LH/CG-R; Luteinizing hormone receptor; LSH-R; LHCGR sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: LHCGR siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 700

• Applicable Applications for LHCGR sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse LHCGR gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for LHCGR sirna

siRNA to inhibit LHCGR expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 7305233
• NCBI GeneID: 16867
• NCBI Accession #: NP_038610.1
• NCBI GenBank Nucleotide #: NM_013582.2
• UniProt Accession #: P30730
• Molecular Weight: 78,215 Da
• NCBI Official Full Name: lutropin-choriogonadotropic hormone receptor
• NCBI Official Synonym Full Names: luteinizing hormone/choriogonadotropin receptor
• NCBI Official Symbol: Lhcgr
• NCBI Official Synonym Symbols: Lhr; LH-R; Gpcr19-rs1
• NCBI Protein Information: lutropin-choriogonadotropic hormone receptor
• UniProt Protein Name: Lutropin-choriogonadotropic hormone receptor
• Protein Family: Lutropin-choriogonadotropic hormone receptor
• UniProt Gene Name: Lhcgr
• UniProt Synonym Gene Names: Lhr; LH/CG-R; LSH-R
• UniProt Entry Name: LSHR_MOUSE

Uniprot Description

LHR: Receptor for lutropin-choriogonadotropic hormone. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Defects in LHCGR are a cause of familial male precocious puberty (FMPP); also known as testotoxicosis. In FMPP the receptor is constitutively activated. Defects in LHCGR are the cause of luteinizing hormone resistance (LHR); also known as Leydig cell hypoplasia in males. LHR is an autosomal recessive disorder characterized by unresponsiveness to luteinizing hormone, defective sexual development in males, and defective follicular development and ovulation, amenorrhea and infertility in females. Two forms of the disorder have been defined in males. Type 1 is a severe form characterized by complete 46,XY male pseudohermaphroditism, low testosterone and high luteinizing hormone levels, total lack of responsiveness to luteinizing and chorionic gonadotropin hormones, lack of breast development, and absent development of secondary male sex characteristics. Type 2, a milder form, displays a broader range of phenotypic expression ranging from micropenis to severe hypospadias. Belongs to the G-protein coupled receptor 1 family. FSH/LSH/TSH subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, GPCR; GPCR, family 1; Membrane protein, multi-pass; Membrane protein, integral

Cellular Component: extracellular space; membrane; endoplasmic reticulum; lysosome; integral to plasma membrane; cytoplasm; integral to membrane; plasma membrane; nucleus; receptor complex; endosome

Molecular Function: lutropin-choriogonadotropic hormone receptor activity; G-protein coupled receptor activity; identical protein binding; signal transducer activity; protein homodimerization activity; peptide hormone binding; protein-hormone receptor activity; peptide receptor activity, G-protein coupled; ATPase binding

Biological Process: protein targeting to lysosome; hormone-mediated signaling; male gonad development; adenylate cyclase activation; positive regulation of inositol trisphosphate biosynthetic process; positive regulation of calcium-mediated signaling; luteinizing hormone signaling pathway; uterus development; signal transduction; arachidonic acid secretion; ovulation cycle process; G-protein signaling, coupled to cAMP nucleotide second messenger; G-protein coupled receptor protein signaling pathway; ovarian follicle development; positive regulation of hormone biosynthetic process; development of secondary male sexual characteristics; spermatogenesis; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of release of sequestered calcium ion into cytosol; cognition; female gonad development