RHO sirna

RHO siRNA (Human)

Cat. Num. AAA828797

• Gene Names: RHO; RP4; OPN2; CSNBAD1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RHO; RHO siRNA (Human); OPN2; Rhodopsin; Opsin-2; RHO sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: RHO siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 348

• Applicable Applications for RHO sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RHO gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RHO sirna

siRNA to inhibit RHO expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4506527
• NCBI GeneID: 6010
• NCBI Accession #: NP_000530.1
• NCBI GenBank Nucleotide #: NM_000539.3
• UniProt Accession #: P08100; Q16414; Q2M249
• Molecular Weight: 38,893 Da
• NCBI Official Full Name: rhodopsin
• NCBI Official Synonym Full Names: rhodopsin
• NCBI Official Symbol: RHO
• NCBI Official Synonym Symbols: RP4; OPN2; CSNBAD1
• NCBI Protein Information: rhodopsin
• UniProt Protein Name: Rhodopsin
• Protein Family: Rhotekin
• UniProt Gene Name: RHO
• UniProt Synonym Gene Names: OPN2
• UniProt Entry Name: OPSD_HUMAN

NCBI Description

Retinitis pigmentosa is an inherited progressive disease which is a major cause of blindness in western communities. It can be inherited as an autosomal dominant, autosomal recessive, or X-linked recessive disorder. In the autosomal dominant form,which comprises about 25% of total cases, approximately 30% of families have mutations in the gene encoding the rod photoreceptor-specific protein rhodopsin. This is the transmembrane protein which, when photoexcited, initiates the visual transduction cascade. Defects in this gene are also one of the causes of congenital stationary night blindness. [provided by RefSeq, Jul 2008]

Uniprot Description

Rhodopsin: a G-protein coupled receptor. The light-absorbing visual pigment in Rod photoreceptor cells. Mediates vision in dim light. Consists of the apoprotein, opsin, covalently linked to cis-retinal. Defects in RHO are a cause of autosomal retinitis pigmentosa and congenital stationary night blindness.

Protein type: Receptor, GPCR; Membrane protein, multi-pass; GPCR, family 1; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q21-q24

Cellular Component: Golgi membrane; Golgi apparatus; photoreceptor outer segment; photoreceptor inner segment; integral to plasma membrane; plasma membrane; rough endoplasmic reticulum membrane; intercellular junction

Molecular Function: G-protein coupled receptor activity; protein binding; spectrin binding; metal ion binding; photoreceptor activity; retinal binding

Biological Process: rhodopsin mediated signaling; phototransduction, visible light; G-protein coupled receptor protein signaling pathway; red, far-red light phototransduction; regulation of rhodopsin mediated signaling; visual perception; retina development in camera-type eye; organelle organization and biogenesis; retinoid metabolic process; protein-chromophore linkage; protein amino acid phosphorylation

Disease: Fundus Albipunctatus; Night Blindness, Congenital Stationary, Autosomal Dominant 1; Retinitis Pigmentosa 4