FKBP1A sirna

FKBP1A siRNA (Mouse)

Cat. Num. AAA828748

• Gene Names: Fkbp1a; Fkbp; Fkbp1; FKBP12
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: FKBP1A; FKBP1A siRNA (Mouse); FKBP1; Peptidyl-prolyl cis-trans isomerase FKBP1A; PPIase FKBP1A; 12 kDa FK506-binding protein; 12 kDa FKBP; FKBP-12; Calstabin-1; FK506-binding protein 1A; FKBP-1A; Immunophilin FKBP12; Rotamase; FKBP1A sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: FKBP1A siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 108

• Applicable Applications for FKBP1A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse FKBP1A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Store at -20 degree C for one year.

Related Product Information for FKBP1A sirna

siRNA to inhibit FKBP1A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 689972333
• NCBI GeneID: 14225
• NCBI Accession #: NP_001289006.1
• NCBI GenBank Nucleotide #: NM_001302077.1
• UniProt Accession #: P26883; Q545E9
• NCBI Official Full Name: peptidyl-prolyl cis-trans isomerase FKBP1A isoform 1
• NCBI Official Synonym Full Names: FK506 binding protein 1a
• NCBI Official Symbol: Fkbp1a
• NCBI Official Synonym Symbols: Fkbp; Fkbp1; FKBP12
• NCBI Protein Information: peptidyl-prolyl cis-trans isomerase FKBP1A
• UniProt Protein Name: Peptidyl-prolyl cis-trans isomerase FKBP1A
• Protein Family: Peptidyl-prolyl cis-trans isomerase
• UniProt Gene Name: Fkbp1a
• UniProt Synonym Gene Names: Fkbp1; PPIase FKBP1A; 12 kDa FKBP; FKBP-12; FKBP-1A
• UniProt Entry Name: FKB1A_MOUSE

NCBI Description

This gene is a member of the immunophilin family. The encoded protein is a cis-trans prolyl isomerase that binds the immunosuppressants FK506 and rapamycin, and is associated with immunoregulation, protein folding, receptor signaling, protein trafficking and T-cell activation. It may modulate the calcium release activity of the ryanodine receptor Ryr1. It also interacts with the type I TGF-beta receptor. Disruption of this gene in mouse causes severe ventricular defects. Pseudogenes of this gene have been defined on chromosomes 4, 10, 14, and 16. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2014]

Uniprot Description

FKBP12: an immunophilin and peptidyl-prolyl cis-trans isomerase that binds to the immunosuppressant drugs FK506 (a.k.a. tacrolimus or Fujimycin) and rapamycin (a.k.a. Sirolimus). The FKBP12/rapamycin complex binds NFAT transcription factors and inhibits the induction of T cell genes including IL-2, IL-3, IL-4, TNF-alpha and GM-CSF. FK506 is used in treating patients after organ transplant and those suffering from autoimmune disorders. The immunosuppressant activity of FKBP12 is not apparently related to its prolyl isomerase activity. The FKBP12/rapamycin complex inhibits the mammalian target of rapamycin (mTOR) pathway by directly binding the mTOR Complex1 (mTORC1). mTORC1 contains Raptor, mLST8, and PRAS40, and interacts with DEPTOR, which inhibits its activity. mTOR, a S/T protein kinase, is a central regulator of cellular growth and metabolism. Its inhibition enhances the dependence on aerobic glycolysis in leukemic cells.

Protein type: Isomerase; EC 5.2.1.8

Cellular Component: extracellular matrix; sarcoplasmic reticulum membrane; membrane; intracellular membrane-bound organelle; cell; cytoplasm; nerve terminal; intracellular; cytosol; Z disc

Molecular Function: peptidyl-prolyl cis-trans isomerase activity; protein homodimerization activity; FK506 binding; drug binding; isomerase activity; Hsp70 protein binding; signal transducer activity; protein binding; enzyme binding; activin binding; calcium channel inhibitor activity; SMAD binding; transforming growth factor beta receptor binding

Biological Process: heart morphogenesis; regulation of immune response; positive regulation of I-kappaB kinase/NF-kappaB cascade; protein peptidyl-prolyl isomerization; positive regulation of protein binding; protein folding; cytokine and chemokine mediated signaling pathway; regulation of activin receptor signaling pathway; SMAD protein complex assembly; response to caffeine; negative regulation of protein phosphatase type 2B activity; T cell proliferation; regulation of protein localization; muscle contraction; negative regulation of protein amino acid phosphorylation; positive regulation of protein ubiquitination; release of sequestered calcium ion into cytosol; negative regulation of release of sequestered calcium ion into cytosol; fibril organization and biogenesis; ventricular cardiac muscle morphogenesis