UBASH3B sirna

UBASH3B siRNA (Human)

Cat. Num. AAA828536

• Gene Names: UBASH3B; p70; STS1; STS-1; TULA2; TULA-2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: UBASH3B; UBASH3B siRNA (Human); KIAA1959; STS1; Ubiquitin-associated and SH3 domain-containing protein B; Cbl-interacting protein p70; Suppressor of T-cell receptor signaling 1; STS-1; T-cell ubiquitin ligand 2; TULA-2; Tyrosine-protein phosphatase STS1/TULA2; UBASH3B sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: UBASH3B siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 649

• Applicable Applications for UBASH3B sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human UBASH3B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for UBASH3B sirna

siRNA to inhibit UBASH3B expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 24497612
• NCBI GeneID: 84959
• NCBI Accession #: NP_116262.2
• NCBI GenBank Nucleotide #: NM_032873.4
• UniProt Accession #: Q8TF42; Q53GT5; Q53GT8; Q8NBV7; Q96IG9; Q96NZ2
• Molecular Weight: 72,696 Da
• NCBI Official Full Name: ubiquitin-associated and SH3 domain-containing protein B
• NCBI Official Synonym Full Names: ubiquitin associated and SH3 domain containing B
• NCBI Official Symbol: UBASH3B
• NCBI Official Synonym Symbols: p70; STS1; STS-1; TULA2; TULA-2
• NCBI Protein Information: ubiquitin-associated and SH3 domain-containing protein B
• UniProt Protein Name: Ubiquitin-associated and SH3 domain-containing protein B
• Protein Family: Ubiquitin-associated and SH3 domain-containing protein
• UniProt Gene Name: UBASH3B
• UniProt Synonym Gene Names: KIAA1959; STS1; STS-1; TULA-2
• UniProt Entry Name: UBS3B_HUMAN

NCBI Description

This gene encodes a protein that contains a ubiquitin associated domain at the N-terminus, an SH3 domain, and a C-terminal domain with similarities to the catalytic motif of phosphoglycerate mutase. The encoded protein was found to inhibit endocytosis of epidermal growth factor receptor (EGFR) and platelet-derived growth factor receptor. [provided by RefSeq, Jul 2008]

Uniprot Description

STS-1: Interferes with CBL-mediated down-regulation and degradation of receptor-type tyrosine kinases. Promotes accumulation of activated target receptors, such as T-cell receptors and EGFR, on the cell surface. Exhibits tyrosine phosphatase activity toward several substrates including EGFR, FAK, SYK, and ZAP70. Down-regulates proteins that are dually modified by both protein tyrosine phosphorylation and ubiquitination.

Protein type: EC 3.1.3.48; Protein phosphatase, tyrosine (non-receptor)

Chromosomal Location of Human Ortholog: 11q24.1

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; protein tyrosine phosphatase activity

Biological Process: negative regulation of protein kinase activity; regulation of release of sequestered calcium ion into cytosol