SH3KBP1 sirna

SH3KBP1 siRNA (Rat)

Cat. Num. AAA828451

• Gene Names: Sh3kbp1; Seta
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SH3KBP1; SH3KBP1 siRNA (Rat); RUK; SETA; SH3 domain-containing kinase-binding protein 1; Regulator of ubiquitous kinase; Ruk; SH3-containing. expressed in tumorigenic astrocytes; SH3KBP1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: SH3KBP1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 665

• Applicable Applications for SH3KBP1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat SH3KBP1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SH3KBP1 sirna

siRNA to inhibit SH3KBP1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 29789297
• NCBI GeneID: 84357
• NCBI Accession #: NP_445812.1
• NCBI GenBank Nucleotide #: NM_053360.2
• UniProt Accession #: Q925Q9; Q925R0; Q925R1; Q925R2; Q925R3; Q9JKQ0; Q9JKQ1; Q9JLU9
• Molecular Weight: 64,847 Da
• NCBI Official Full Name: SH3 domain-containing kinase-binding protein 1
• NCBI Official Synonym Full Names: SH3-domain kinase binding protein 1
• NCBI Official Symbol: Sh3kbp1
• NCBI Official Synonym Symbols: Seta
• NCBI Protein Information: SH3 domain-containing kinase-binding protein 1
• UniProt Protein Name: SH3 domain-containing kinase-binding protein 1
• Protein Family: SH3KBP1-binding protein
• UniProt Gene Name: Sh3kbp1
• UniProt Synonym Gene Names: Ruk; Seta; Ruk
• UniProt Entry Name: SH3K1_RAT

NCBI Description

binds apoptosis-linked gene 2 (ALG-2) interacting protein 1; involved in regulation of apoptosis in astrocytes [RGD, Feb 2006]

Uniprot Description

SH3KBP1: Adapter protein involved in regulating diverse signal transduction pathways. Involved in the regulation of endocytosis and lysosomal degradation of ligand-induced receptor tyrosine kinases, including EGFR and MET/hepatocyte growth factor receptor, through a association with CBL and endophilins. The association with CBL, and thus the receptor internalization, may inhibited by an interaction with PDCD6IP and/or SPRY2. Involved in regulation of ligand-dependent endocytosis of the IgE receptor. Attenuates phosphatidylinositol 3-kinase activity by interaction with its regulatory subunit. May be involved in regulation of cell adhesion; promotes the interaction between TTK2B and PDCD6IP. May be involved in the regulation of cellular stress response via the MAPK pathways through its interaction with MAP3K4. Is involved in modulation of tumor necrosis factor mediated apoptosis. Can self-associate and form homotetramers. Interacts with CD2, F-actin capping protein, PIK3R3, GRB2, EGFR, MET, BLNK, MAP3K4, PDCD6IP, SPRY2, ARHGAP17, ARHGAP27, MAGI2, CRK, BCAR1, SOS1, ASAP1, ARAP3, HIP1R, SYNJ2, INPP5D and STAP1. Interacts with CBL and CBLB, but does not interact with CBLC. Two molecules of SH3KBP1 seem to bind through their respective SH3 1 domain to one molecule of CBLB. The interaction with CBL or CBLB and EGFR is increased upon EGF stimulation. The interaction with CBL is attenuated by PDCD6IP. Interacts through its proline-rich region with the SH3 domain of endophilins SH3GL1, SH3GL2 and SH3GL3. The SH3KBP1-endophilin complex seems to associate with a complex containing the phosphorylated receptor (EGFR or MET) and phosphorylated CBL. Probably associates with ASAP1 and phosphorylated EGFR. Probably part of a complex consisting of at least SH3KBP1, ASAP1 and ARAP3. Interacts with focal adhesion kinases PTK2 AND PTK2B, probably as a dimer. Interacts with DAB2 and probably associates with chathrin through its interaction with DAB2. Part of a complex consisting of SH3KBP1, DAB2, and clathrin heavy chain. DAB2 and clathrin dissociate from SH3KBP1 following growth factor treatment, enabling interaction with CBL. Interacts with DDN and probably associates with MAGI2 through its interaction with DDN. Interacts with the SH3 domains of SRC tyrosine-protein kinases SRC, LCK, LYN, FGR, FYN and HCK. Interacts with TRADD, BIRC2, TRAF1, TRAF2 and TNFR1, and the association with a TNFR1-associated complex upon stimulation with TNF-alpha seems to be mediated by SRC. Probably interacts with SH3KBP1. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis; Apoptosis

Cellular Component: neuron projection; cytoskeleton; cytoplasmic vesicle membrane; focal adhesion; synapse; intercellular junction

Molecular Function: protein binding; SH3 domain binding

Biological Process: regulation of apoptosis; regulation of cell shape; cell migration; apoptosis; endocytosis; cytoskeleton organization and biogenesis